Understanding single enzyme activity via the nano-impact technique
To evaluate the possible detection of single enzyme activity via electrochemical methods, a combined finite difference and random walk simulation is used to model individual enzyme-electrode collisions where such events are monitored amperometrically via the measurement of products formed by the enz...
| Main Authors: | , , , |
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| Format: | Journal article |
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Royal Society of Chemistry
2017
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| _version_ | 1826258728245002240 |
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| author | Lin, C Kätelhön, E Sepunaru, L Compton, R |
| author_facet | Lin, C Kätelhön, E Sepunaru, L Compton, R |
| author_sort | Lin, C |
| collection | OXFORD |
| description | To evaluate the possible detection of single enzyme activity via electrochemical methods, a combined finite difference and random walk simulation is used to model individual enzyme-electrode collisions where such events are monitored amperometrically via the measurement of products formed by the enzyme in solution. It is found that the observed signal is highly sensitive to both the enzyme turnover number, the size of the electrode and the bandwidth of the electronics. Taking single catalase impacts as an example, simulation results are compared with experimental data. Our work shows the requirement for the detection of electrochemically active product formed by individual enzymes and gives guidance for the design of experiments. |
| first_indexed | 2024-03-06T18:38:33Z |
| format | Journal article |
| id | oxford-uuid:0c201f6b-8944-4a70-929f-cc0bab96cb43 |
| institution | University of Oxford |
| last_indexed | 2024-03-06T18:38:33Z |
| publishDate | 2017 |
| publisher | Royal Society of Chemistry |
| record_format | dspace |
| spelling | oxford-uuid:0c201f6b-8944-4a70-929f-cc0bab96cb432022-03-26T09:33:12ZUnderstanding single enzyme activity via the nano-impact techniqueJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:0c201f6b-8944-4a70-929f-cc0bab96cb43Symplectic Elements at OxfordRoyal Society of Chemistry2017Lin, CKätelhön, ESepunaru, LCompton, RTo evaluate the possible detection of single enzyme activity via electrochemical methods, a combined finite difference and random walk simulation is used to model individual enzyme-electrode collisions where such events are monitored amperometrically via the measurement of products formed by the enzyme in solution. It is found that the observed signal is highly sensitive to both the enzyme turnover number, the size of the electrode and the bandwidth of the electronics. Taking single catalase impacts as an example, simulation results are compared with experimental data. Our work shows the requirement for the detection of electrochemically active product formed by individual enzymes and gives guidance for the design of experiments. |
| spellingShingle | Lin, C Kätelhön, E Sepunaru, L Compton, R Understanding single enzyme activity via the nano-impact technique |
| title | Understanding single enzyme activity via the nano-impact technique |
| title_full | Understanding single enzyme activity via the nano-impact technique |
| title_fullStr | Understanding single enzyme activity via the nano-impact technique |
| title_full_unstemmed | Understanding single enzyme activity via the nano-impact technique |
| title_short | Understanding single enzyme activity via the nano-impact technique |
| title_sort | understanding single enzyme activity via the nano impact technique |
| work_keys_str_mv | AT linc understandingsingleenzymeactivityviathenanoimpacttechnique AT katelhone understandingsingleenzymeactivityviathenanoimpacttechnique AT sepunarul understandingsingleenzymeactivityviathenanoimpacttechnique AT comptonr understandingsingleenzymeactivityviathenanoimpacttechnique |