Crystallization and preliminary crystallographic analysis of deoxyuridine 5'-triphosphate nucleotidohydrolase from Bacillus subtilis.
Single crystals of purified homotrimeric deoxyuridine 5'-triphosphate nucleotidohydrolase (dUTPase) from Bacillus subtilis have been grown under several different conditions using vapour diffusion. X-ray diffraction data have been collected using synchrotron radiation from three crystal forms o...
| Main Authors: | , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
2001
|
| _version_ | 1797053075313131520 |
|---|---|
| author | Persson, R Harkiolaki, M McGeehan, J Wilson, K |
| author_facet | Persson, R Harkiolaki, M McGeehan, J Wilson, K |
| author_sort | Persson, R |
| collection | OXFORD |
| description | Single crystals of purified homotrimeric deoxyuridine 5'-triphosphate nucleotidohydrolase (dUTPase) from Bacillus subtilis have been grown under several different conditions using vapour diffusion. X-ray diffraction data have been collected using synchrotron radiation from three crystal forms of the unliganded enzyme and from enzyme cocrystallized with a substrate analogue and inhibitor, dUDP, and a metal ion, Sr(2+). The three crystal forms of unliganded enzyme belong to hexagonal (P6(3)), orthorhombic (P2(1)2(1)2) and cubic (P2(1)3) space groups and data have been recorded to 1.75, 1.90 and 2.50 A spacing, respectively. Crystals grown in the presence of dUDP and Sr(2+) belong to the orthorhombic space group P2(1)2(1)2(1) and data were measured to 1.90 A spacing. Solution of the hexagonal crystal form by molecular replacement using the dUTPase from feline immunodeficiency virus as a search model is in progress. |
| first_indexed | 2024-03-06T18:38:57Z |
| format | Journal article |
| id | oxford-uuid:0c3d9955-9490-4e07-b5b9-c47df67ddc9a |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T18:38:57Z |
| publishDate | 2001 |
| record_format | dspace |
| spelling | oxford-uuid:0c3d9955-9490-4e07-b5b9-c47df67ddc9a2022-03-26T09:33:50ZCrystallization and preliminary crystallographic analysis of deoxyuridine 5'-triphosphate nucleotidohydrolase from Bacillus subtilis.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:0c3d9955-9490-4e07-b5b9-c47df67ddc9aEnglishSymplectic Elements at Oxford2001Persson, RHarkiolaki, MMcGeehan, JWilson, KSingle crystals of purified homotrimeric deoxyuridine 5'-triphosphate nucleotidohydrolase (dUTPase) from Bacillus subtilis have been grown under several different conditions using vapour diffusion. X-ray diffraction data have been collected using synchrotron radiation from three crystal forms of the unliganded enzyme and from enzyme cocrystallized with a substrate analogue and inhibitor, dUDP, and a metal ion, Sr(2+). The three crystal forms of unliganded enzyme belong to hexagonal (P6(3)), orthorhombic (P2(1)2(1)2) and cubic (P2(1)3) space groups and data have been recorded to 1.75, 1.90 and 2.50 A spacing, respectively. Crystals grown in the presence of dUDP and Sr(2+) belong to the orthorhombic space group P2(1)2(1)2(1) and data were measured to 1.90 A spacing. Solution of the hexagonal crystal form by molecular replacement using the dUTPase from feline immunodeficiency virus as a search model is in progress. |
| spellingShingle | Persson, R Harkiolaki, M McGeehan, J Wilson, K Crystallization and preliminary crystallographic analysis of deoxyuridine 5'-triphosphate nucleotidohydrolase from Bacillus subtilis. |
| title | Crystallization and preliminary crystallographic analysis of deoxyuridine 5'-triphosphate nucleotidohydrolase from Bacillus subtilis. |
| title_full | Crystallization and preliminary crystallographic analysis of deoxyuridine 5'-triphosphate nucleotidohydrolase from Bacillus subtilis. |
| title_fullStr | Crystallization and preliminary crystallographic analysis of deoxyuridine 5'-triphosphate nucleotidohydrolase from Bacillus subtilis. |
| title_full_unstemmed | Crystallization and preliminary crystallographic analysis of deoxyuridine 5'-triphosphate nucleotidohydrolase from Bacillus subtilis. |
| title_short | Crystallization and preliminary crystallographic analysis of deoxyuridine 5'-triphosphate nucleotidohydrolase from Bacillus subtilis. |
| title_sort | crystallization and preliminary crystallographic analysis of deoxyuridine 5 triphosphate nucleotidohydrolase from bacillus subtilis |
| work_keys_str_mv | AT perssonr crystallizationandpreliminarycrystallographicanalysisofdeoxyuridine5triphosphatenucleotidohydrolasefrombacillussubtilis AT harkiolakim crystallizationandpreliminarycrystallographicanalysisofdeoxyuridine5triphosphatenucleotidohydrolasefrombacillussubtilis AT mcgeehanj crystallizationandpreliminarycrystallographicanalysisofdeoxyuridine5triphosphatenucleotidohydrolasefrombacillussubtilis AT wilsonk crystallizationandpreliminarycrystallographicanalysisofdeoxyuridine5triphosphatenucleotidohydrolasefrombacillussubtilis |