Alterations of VLDL composition during alimentary lipemia.
Apoliprotein (apo) B-100-containing very low density lipoprotein (VLDL) particles secreted from the liver accumulate in plasma during alimentary lipemia. To determine whether changes of VLDL composition occur in the postprandial state that may render these lipoproteins more atherogenic, apoE, C-I, C...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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1997
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| _version_ | 1797053102272020480 |
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| author | Björkegren, J Hamsten, A Milne, R Karpe, F |
| author_facet | Björkegren, J Hamsten, A Milne, R Karpe, F |
| author_sort | Björkegren, J |
| collection | OXFORD |
| description | Apoliprotein (apo) B-100-containing very low density lipoprotein (VLDL) particles secreted from the liver accumulate in plasma during alimentary lipemia. To determine whether changes of VLDL composition occur in the postprandial state that may render these lipoproteins more atherogenic, apoE, C-I, C-II, and C-III, and lipids (triglycerides, phospholipids, and cholesterol) were measured in Svedberg flotation (Sf) 60-400 (large) and Sf 20-60 (small) VLDL before and after an oral fat load. Ten normotriglyceridemic (NTG) and three hypertriglyceridemic (HTG) healthy men were given a fat-rich mixed meal (1,000 kCal with 60.2 E% from fat). Triglyceride-rich lipoproteins were isolated by density gradient ultracentrifugation from plasma samples obtained before (fasting) and at 2-h intervals after the meal. VLDL was then separated from chylomicrons and their remnants by immunoaffinity chromatography using monoclonal antibodies 4G3 and 5E11, recognizing apoB-100, but not apoB-48 epitopes. Large and small VLDL isolated from the NTG group were enriched with apoE and C-I, and cholesterol, but depleted of apoC-II in the postprandial state, whereas the apoC-III, triglyceride, and phospholipid contents were essentially unchanged. The compositional changes of VLDL in HTG subjects were similar but more pronounced compared with NTG subjects. We conclude that postprandial lipemia in healthy men induces transient compositional alterations of VLDL that link these lipoprotein species to the formation of atherosclerosis. |
| first_indexed | 2024-03-06T18:39:22Z |
| format | Journal article |
| id | oxford-uuid:0c5c7fb0-8418-4430-9b2c-0f244a5a63b4 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T18:39:22Z |
| publishDate | 1997 |
| record_format | dspace |
| spelling | oxford-uuid:0c5c7fb0-8418-4430-9b2c-0f244a5a63b42022-03-26T09:34:31ZAlterations of VLDL composition during alimentary lipemia.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:0c5c7fb0-8418-4430-9b2c-0f244a5a63b4EnglishSymplectic Elements at Oxford1997Björkegren, JHamsten, AMilne, RKarpe, FApoliprotein (apo) B-100-containing very low density lipoprotein (VLDL) particles secreted from the liver accumulate in plasma during alimentary lipemia. To determine whether changes of VLDL composition occur in the postprandial state that may render these lipoproteins more atherogenic, apoE, C-I, C-II, and C-III, and lipids (triglycerides, phospholipids, and cholesterol) were measured in Svedberg flotation (Sf) 60-400 (large) and Sf 20-60 (small) VLDL before and after an oral fat load. Ten normotriglyceridemic (NTG) and three hypertriglyceridemic (HTG) healthy men were given a fat-rich mixed meal (1,000 kCal with 60.2 E% from fat). Triglyceride-rich lipoproteins were isolated by density gradient ultracentrifugation from plasma samples obtained before (fasting) and at 2-h intervals after the meal. VLDL was then separated from chylomicrons and their remnants by immunoaffinity chromatography using monoclonal antibodies 4G3 and 5E11, recognizing apoB-100, but not apoB-48 epitopes. Large and small VLDL isolated from the NTG group were enriched with apoE and C-I, and cholesterol, but depleted of apoC-II in the postprandial state, whereas the apoC-III, triglyceride, and phospholipid contents were essentially unchanged. The compositional changes of VLDL in HTG subjects were similar but more pronounced compared with NTG subjects. We conclude that postprandial lipemia in healthy men induces transient compositional alterations of VLDL that link these lipoprotein species to the formation of atherosclerosis. |
| spellingShingle | Björkegren, J Hamsten, A Milne, R Karpe, F Alterations of VLDL composition during alimentary lipemia. |
| title | Alterations of VLDL composition during alimentary lipemia. |
| title_full | Alterations of VLDL composition during alimentary lipemia. |
| title_fullStr | Alterations of VLDL composition during alimentary lipemia. |
| title_full_unstemmed | Alterations of VLDL composition during alimentary lipemia. |
| title_short | Alterations of VLDL composition during alimentary lipemia. |
| title_sort | alterations of vldl composition during alimentary lipemia |
| work_keys_str_mv | AT bjorkegrenj alterationsofvldlcompositionduringalimentarylipemia AT hamstena alterationsofvldlcompositionduringalimentarylipemia AT milner alterationsofvldlcompositionduringalimentarylipemia AT karpef alterationsofvldlcompositionduringalimentarylipemia |