High frequency, sustained T cell responses to PARV4 suggest viral persistence in vivo
<p>Background. Parvovirus 4 (PARV4) is a recently identified human virus that has been found in livers of patients infected with hepatitis C virus (HCV) and in bone marrow of individuals infected with human immunodeficiency virus (HIV). T cells are important in controlling viruses but may also...
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| Format: | Journal article |
| Language: | English |
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Oxford University Press
2011
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| author | Simmons, R Sharp, C Sims, S Kloverpris, H Goulder, P Simmonds, P Bowness, P Klenerman, P |
| author2 | Infectious Diseases Society of America |
| author_facet | Infectious Diseases Society of America Simmons, R Sharp, C Sims, S Kloverpris, H Goulder, P Simmonds, P Bowness, P Klenerman, P |
| author_sort | Simmons, R |
| collection | OXFORD |
| description | <p>Background. Parvovirus 4 (PARV4) is a recently identified human virus that has been found in livers of patients infected with hepatitis C virus (HCV) and in bone marrow of individuals infected with human immunodeficiency virus (HIV). T cells are important in controlling viruses but may also contribute to disease pathogenesis. The interaction of PARV4 with the cellular immune system has not been described. Consequently, we investigated whether T cell responses to PARV4 could be detected in individuals exposed to blood-borne viruses.</p><p>Methods. Interferon γ (INF-γ) enzyme-linked immunospot assay, intracellular cytokine staining, and a tetrameric HLA-A*0201-peptide complex were used to define the lymphocyte populations responding to PARV4 NS peptides in 88 HCV-positive and 13 HIV-positive individuals. Antibody responses were tested using a recently developed PARV4 enzyme-linked immunosorbent assay.</p><p>Results. High-frequency T cell responses against multiple PARV4 NS peptides and antibodies were observed in 26% of individuals. Typical responses to the NS pools were > 1000 spot-forming units per million peripheral blood mononuclear cells.</p><p>Conclusions. PARV4 infection is common in individuals exposed to blood-borne viruses and elicits strong T cell responses, a feature typically associated with persistent, contained infections such as cytomegalovirus. Persistence of PARV4 viral antigen in tissue from HVC-positive and HIV-positive individuals and/or the associated antiviral T cell response may contribute to disease pathogenesis.</p> |
| first_indexed | 2024-03-06T18:39:55Z |
| format | Journal article |
| id | oxford-uuid:0c85ea7b-8927-41e2-9cde-c5a7d0fd74a3 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T18:39:55Z |
| publishDate | 2011 |
| publisher | Oxford University Press |
| record_format | dspace |
| spelling | oxford-uuid:0c85ea7b-8927-41e2-9cde-c5a7d0fd74a32022-03-26T09:35:32ZHigh frequency, sustained T cell responses to PARV4 suggest viral persistence in vivoJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:0c85ea7b-8927-41e2-9cde-c5a7d0fd74a3HIV/AIDSInfectious diseasesEnglishOxford University Research Archive - ValetOxford University Press2011Simmons, RSharp, CSims, SKloverpris, HGoulder, PSimmonds, PBowness, PKlenerman, PInfectious Diseases Society of AmericaHIV Medicine Association<p>Background. Parvovirus 4 (PARV4) is a recently identified human virus that has been found in livers of patients infected with hepatitis C virus (HCV) and in bone marrow of individuals infected with human immunodeficiency virus (HIV). T cells are important in controlling viruses but may also contribute to disease pathogenesis. The interaction of PARV4 with the cellular immune system has not been described. Consequently, we investigated whether T cell responses to PARV4 could be detected in individuals exposed to blood-borne viruses.</p><p>Methods. Interferon γ (INF-γ) enzyme-linked immunospot assay, intracellular cytokine staining, and a tetrameric HLA-A*0201-peptide complex were used to define the lymphocyte populations responding to PARV4 NS peptides in 88 HCV-positive and 13 HIV-positive individuals. Antibody responses were tested using a recently developed PARV4 enzyme-linked immunosorbent assay.</p><p>Results. High-frequency T cell responses against multiple PARV4 NS peptides and antibodies were observed in 26% of individuals. Typical responses to the NS pools were > 1000 spot-forming units per million peripheral blood mononuclear cells.</p><p>Conclusions. PARV4 infection is common in individuals exposed to blood-borne viruses and elicits strong T cell responses, a feature typically associated with persistent, contained infections such as cytomegalovirus. Persistence of PARV4 viral antigen in tissue from HVC-positive and HIV-positive individuals and/or the associated antiviral T cell response may contribute to disease pathogenesis.</p> |
| spellingShingle | HIV/AIDS Infectious diseases Simmons, R Sharp, C Sims, S Kloverpris, H Goulder, P Simmonds, P Bowness, P Klenerman, P High frequency, sustained T cell responses to PARV4 suggest viral persistence in vivo |
| title | High frequency, sustained T cell responses to PARV4 suggest viral persistence in vivo |
| title_full | High frequency, sustained T cell responses to PARV4 suggest viral persistence in vivo |
| title_fullStr | High frequency, sustained T cell responses to PARV4 suggest viral persistence in vivo |
| title_full_unstemmed | High frequency, sustained T cell responses to PARV4 suggest viral persistence in vivo |
| title_short | High frequency, sustained T cell responses to PARV4 suggest viral persistence in vivo |
| title_sort | high frequency sustained t cell responses to parv4 suggest viral persistence in vivo |
| topic | HIV/AIDS Infectious diseases |
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