Characterisation of endometrial B cells and lymphocytes in the peri-implantation window
<p>Immunological regulation at the implantation site is a complex process, dysfunction may contribute to infertility, recurrent implantation failure (RIF) or recurrent pregnancy loss (RPL). Apart from endometrial NK and T cells, whose phenotypic profile and functional involvement have been rec...
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
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2021
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| _version_ | 1826315605383315456 |
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| author | Shen, M |
| author2 | Granne, I |
| author_facet | Granne, I Shen, M |
| author_sort | Shen, M |
| collection | OXFORD |
| description | <p>Immunological regulation at the implantation site is a complex process, dysfunction may contribute to infertility, recurrent implantation failure (RIF) or recurrent pregnancy loss (RPL). Apart from endometrial NK and T cells, whose phenotypic profile and functional involvement have been recognised, evidence suggests that endometrial B cells may also
have a potential role in reproductive pathologies. This thesis aimed to investigate endometrial immune profiles during human implantation, with a specific focus on the role of endometrial B cells.</p>
<p>Both healthy women and infertility, RIF or RPL patients were recruited for endometrial immune profiling, focusing on cell proportions (NK, T and B cells) and activation status. Additionally, peripheral blood samples were collected and analysed in parallel. Significant
differences were observed between peripheral and endometrial immune profiles, in minor cell populations like CD56<sup>bright</sup> CD16<sup>dim</sup> NK cells and CD4 CD8 double positive T cells, such differences were not previously reported. A standard operating protocol (SOP) was established so that comparison between controls and infertility, RIF or RPL patients
could be made with more patients being enrolled in the near future.</p>
<p>The role of B cells in the human endometrium was investigated. A systematic review was performed to understand the B cell involvement in reproductive pathologies. A number of immunohistochemistry studies were retrieved that identified endometrial B cells in association with T cells in ‘lymphoid aggregates (LAs)’, however, neither have been extensively
characterised. RNA-sequencing and flow cytometric analysis were performed on CD19+ endometrial B cells. B cells comprised 1-5% of endometrial immune cells, the majority were naïve or memory B cells, with few plasma cells. Compared with circulating B cells, endometrial B cells had an activated phenotype, with increased expression of CD69,
HLA-DR, CD74 and CD83, and IL-10 production capacities. PD1+CXCR5+ICOS+ T follicular helper-like cells and FAS+IgD germinal centre-like B cells were present in the endometrium. Together with previously reported LAs, endometrial B cells may be an
active player in shaping the endometrial immune environment.</p> |
| first_indexed | 2024-03-06T18:41:17Z |
| format | Thesis |
| id | oxford-uuid:0cf191b7-1247-479e-a05d-db2ccb797ab2 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-12-09T03:29:16Z |
| publishDate | 2021 |
| record_format | dspace |
| spelling | oxford-uuid:0cf191b7-1247-479e-a05d-db2ccb797ab22024-12-01T12:00:00ZCharacterisation of endometrial B cells and lymphocytes in the peri-implantation windowThesishttp://purl.org/coar/resource_type/c_db06uuid:0cf191b7-1247-479e-a05d-db2ccb797ab2Reproductive healthEnglishHyrax Deposit2021Shen, MGranne, ISouthcombe, J<p>Immunological regulation at the implantation site is a complex process, dysfunction may contribute to infertility, recurrent implantation failure (RIF) or recurrent pregnancy loss (RPL). Apart from endometrial NK and T cells, whose phenotypic profile and functional involvement have been recognised, evidence suggests that endometrial B cells may also have a potential role in reproductive pathologies. This thesis aimed to investigate endometrial immune profiles during human implantation, with a specific focus on the role of endometrial B cells.</p> <p>Both healthy women and infertility, RIF or RPL patients were recruited for endometrial immune profiling, focusing on cell proportions (NK, T and B cells) and activation status. Additionally, peripheral blood samples were collected and analysed in parallel. Significant differences were observed between peripheral and endometrial immune profiles, in minor cell populations like CD56<sup>bright</sup> CD16<sup>dim</sup> NK cells and CD4 CD8 double positive T cells, such differences were not previously reported. A standard operating protocol (SOP) was established so that comparison between controls and infertility, RIF or RPL patients could be made with more patients being enrolled in the near future.</p> <p>The role of B cells in the human endometrium was investigated. A systematic review was performed to understand the B cell involvement in reproductive pathologies. A number of immunohistochemistry studies were retrieved that identified endometrial B cells in association with T cells in ‘lymphoid aggregates (LAs)’, however, neither have been extensively characterised. RNA-sequencing and flow cytometric analysis were performed on CD19+ endometrial B cells. B cells comprised 1-5% of endometrial immune cells, the majority were naïve or memory B cells, with few plasma cells. Compared with circulating B cells, endometrial B cells had an activated phenotype, with increased expression of CD69, HLA-DR, CD74 and CD83, and IL-10 production capacities. PD1+CXCR5+ICOS+ T follicular helper-like cells and FAS+IgD germinal centre-like B cells were present in the endometrium. Together with previously reported LAs, endometrial B cells may be an active player in shaping the endometrial immune environment.</p> |
| spellingShingle | Reproductive health Shen, M Characterisation of endometrial B cells and lymphocytes in the peri-implantation window |
| title | Characterisation of endometrial B cells and lymphocytes in the peri-implantation window |
| title_full | Characterisation of endometrial B cells and lymphocytes in the peri-implantation window |
| title_fullStr | Characterisation of endometrial B cells and lymphocytes in the peri-implantation window |
| title_full_unstemmed | Characterisation of endometrial B cells and lymphocytes in the peri-implantation window |
| title_short | Characterisation of endometrial B cells and lymphocytes in the peri-implantation window |
| title_sort | characterisation of endometrial b cells and lymphocytes in the peri implantation window |
| topic | Reproductive health |
| work_keys_str_mv | AT shenm characterisationofendometrialbcellsandlymphocytesintheperiimplantationwindow |