Proteomic response of the marine ammonia-oxidising archaeon Nitrosopumilus maritimus to iron limitation reveals strategies to compensate for nutrient scarcity
Dissolved iron (Fe) is vanishingly low in the oceans, with ecological success conferred to microorganisms that can restructure their biochemistry to maintain high growth rates during Fe scarcity. Chemolithoautotrophic ammonia-oxidising archaea (AOA) are highly abundant in the oceans, constituting ~3...
| Հիմնական հեղինակներ: | , , , , |
|---|---|
| Ձևաչափ: | Journal article |
| Լեզու: | English |
| Հրապարակվել է: |
Wiley
2021
|
| _version_ | 1826307406435450880 |
|---|---|
| author | Shafiee, RT Snow, JT Hester, S Zhang, Q Rickaby, REM |
| author_facet | Shafiee, RT Snow, JT Hester, S Zhang, Q Rickaby, REM |
| author_sort | Shafiee, RT |
| collection | OXFORD |
| description | Dissolved iron (Fe) is vanishingly low in the oceans, with ecological success conferred to microorganisms that can restructure their biochemistry to maintain high growth rates during Fe scarcity. Chemolithoautotrophic ammonia-oxidising archaea (AOA) are highly abundant in the oceans, constituting ~30% of cells below the photic zone. Here we examine the proteomic response of the AOA isolate Nitrosopumilus maritimus to growth-limiting Fe concentrations. Under Fe limitation, we observed a significant reduction in the intensity of Fe-dense ferredoxins associated with respiratory complex I whilst complex III and IV proteins with more central roles in the electron transport chain remain unchanged. We concomitantly observed an increase in the intensity of Fe-free functional alternatives such as flavodoxin and plastocyanin, thioredoxin and alkyl hydroperoxide which are known to mediate electron transport and reactive oxygen species detoxification, respectively. Under Fe limitation, we found a marked increase in the intensity of the ABC phosphonate transport system (Phn), highlighting an intriguing link between Fe and P cycling in N. maritimus. We hypothesise that an elevated uptake of exogenous phosphonates under Fe limitation may either supplement N. maritimus' endogenous methylphosphonate biosynthesis pathway - which requires Fe - or enhance the production of phosphonate-containing exopolysaccharides known to efficiently bind environmental Fe. |
| first_indexed | 2024-03-07T07:02:39Z |
| format | Journal article |
| id | oxford-uuid:0d534298-fbcc-48f9-9c76-485fff088150 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T07:02:39Z |
| publishDate | 2021 |
| publisher | Wiley |
| record_format | dspace |
| spelling | oxford-uuid:0d534298-fbcc-48f9-9c76-485fff0881502022-04-06T06:43:47ZProteomic response of the marine ammonia-oxidising archaeon Nitrosopumilus maritimus to iron limitation reveals strategies to compensate for nutrient scarcityJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:0d534298-fbcc-48f9-9c76-485fff088150EnglishSymplectic ElementsWiley2021Shafiee, RTSnow, JTHester, SZhang, QRickaby, REMDissolved iron (Fe) is vanishingly low in the oceans, with ecological success conferred to microorganisms that can restructure their biochemistry to maintain high growth rates during Fe scarcity. Chemolithoautotrophic ammonia-oxidising archaea (AOA) are highly abundant in the oceans, constituting ~30% of cells below the photic zone. Here we examine the proteomic response of the AOA isolate Nitrosopumilus maritimus to growth-limiting Fe concentrations. Under Fe limitation, we observed a significant reduction in the intensity of Fe-dense ferredoxins associated with respiratory complex I whilst complex III and IV proteins with more central roles in the electron transport chain remain unchanged. We concomitantly observed an increase in the intensity of Fe-free functional alternatives such as flavodoxin and plastocyanin, thioredoxin and alkyl hydroperoxide which are known to mediate electron transport and reactive oxygen species detoxification, respectively. Under Fe limitation, we found a marked increase in the intensity of the ABC phosphonate transport system (Phn), highlighting an intriguing link between Fe and P cycling in N. maritimus. We hypothesise that an elevated uptake of exogenous phosphonates under Fe limitation may either supplement N. maritimus' endogenous methylphosphonate biosynthesis pathway - which requires Fe - or enhance the production of phosphonate-containing exopolysaccharides known to efficiently bind environmental Fe. |
| spellingShingle | Shafiee, RT Snow, JT Hester, S Zhang, Q Rickaby, REM Proteomic response of the marine ammonia-oxidising archaeon Nitrosopumilus maritimus to iron limitation reveals strategies to compensate for nutrient scarcity |
| title | Proteomic response of the marine ammonia-oxidising archaeon Nitrosopumilus maritimus to iron limitation reveals strategies to compensate for nutrient scarcity |
| title_full | Proteomic response of the marine ammonia-oxidising archaeon Nitrosopumilus maritimus to iron limitation reveals strategies to compensate for nutrient scarcity |
| title_fullStr | Proteomic response of the marine ammonia-oxidising archaeon Nitrosopumilus maritimus to iron limitation reveals strategies to compensate for nutrient scarcity |
| title_full_unstemmed | Proteomic response of the marine ammonia-oxidising archaeon Nitrosopumilus maritimus to iron limitation reveals strategies to compensate for nutrient scarcity |
| title_short | Proteomic response of the marine ammonia-oxidising archaeon Nitrosopumilus maritimus to iron limitation reveals strategies to compensate for nutrient scarcity |
| title_sort | proteomic response of the marine ammonia oxidising archaeon nitrosopumilus maritimus to iron limitation reveals strategies to compensate for nutrient scarcity |
| work_keys_str_mv | AT shafieert proteomicresponseofthemarineammoniaoxidisingarchaeonnitrosopumilusmaritimustoironlimitationrevealsstrategiestocompensatefornutrientscarcity AT snowjt proteomicresponseofthemarineammoniaoxidisingarchaeonnitrosopumilusmaritimustoironlimitationrevealsstrategiestocompensatefornutrientscarcity AT hesters proteomicresponseofthemarineammoniaoxidisingarchaeonnitrosopumilusmaritimustoironlimitationrevealsstrategiestocompensatefornutrientscarcity AT zhangq proteomicresponseofthemarineammoniaoxidisingarchaeonnitrosopumilusmaritimustoironlimitationrevealsstrategiestocompensatefornutrientscarcity AT rickabyrem proteomicresponseofthemarineammoniaoxidisingarchaeonnitrosopumilusmaritimustoironlimitationrevealsstrategiestocompensatefornutrientscarcity |