Fluorescence nanoscopy by ground-state depletion and single-molecule return.

We introduce far-field fluorescence nanoscopy with ordinary fluorophores based on switching the majority of them to a metastable dark state, such as the triplet, and calculating the position of those left or those that spontaneously returned to the ground state. Continuous widefield illumination by...

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Bibliographic Details
Main Authors: Fölling, J, Bossi, M, Bock, H, Medda, R, Wurm, C, Hein, B, Jakobs, S, Eggeling, C, Hell, S
Format: Journal article
Language:English
Published: 2008