Single-molecule kinetics of two-step divalent cation chelation.

A double hug: Two half-chelating ligands were covalently attached within the lumen of a protein nanopore (see picture). By electrical recording, the formation of fully chelated Zn 2+ ions was monitored at the single-molecule level, thus revealing the rate constants for the ei...

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Bibliographic Details
Main Authors: Hammerstein, A, Shin, S, Bayley, H
Format: Journal article
Language:English
Published: 2010
Description
Summary:A double hug: Two half-chelating ligands were covalently attached within the lumen of a protein nanopore (see picture). By electrical recording, the formation of fully chelated Zn 2+ ions was monitored at the single-molecule level, thus revealing the rate constants for the eight major steps in the process. Figure Equotion Present. © 2010 Wiley-VCH Verlag GmbH and Co. KGaA.