3D super-resolution deep-tissue imaging in living mice
Stimulated emission depletion (STED) microscopy enables the three-dimensional (3D) visualization of dynamic nanoscale structures in living cells, offering unique insights into their organization. However, 3D-STED imaging deep inside biological tissue is obstructed by optical aberrations and light sc...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
Optical Society of America
2021
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| _version_ | 1826259255961845760 |
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| author | Velasco, MGM Zhang, M Antonello, J Yuan, P Allgeyer, ES May, D M'Saad, O Kidd, P Barentine, AES Greco, V Grutzendler, J Booth, MJ Bewersdorf, J |
| author_facet | Velasco, MGM Zhang, M Antonello, J Yuan, P Allgeyer, ES May, D M'Saad, O Kidd, P Barentine, AES Greco, V Grutzendler, J Booth, MJ Bewersdorf, J |
| author_sort | Velasco, MGM |
| collection | OXFORD |
| description | Stimulated emission depletion (STED) microscopy enables the three-dimensional (3D) visualization of dynamic nanoscale structures in living cells, offering unique insights into their organization. However, 3D-STED imaging deep inside biological tissue is obstructed by optical aberrations and light scattering. We present a STED system that overcomes these challenges. Through the combination of two-photon excitation, adaptive optics, red-emitting organic dyes, and a long-working-distance water-immersion objective lens, our system achieves aberration-corrected 3D super-resolution imaging, which we demonstrate 164 µm deep in fixed mouse brain tissue and 76 µm deep in the brain of a living mouse. |
| first_indexed | 2024-03-06T18:47:00Z |
| format | Journal article |
| id | oxford-uuid:0edac9fc-e3db-4734-a7a2-17b34213cdb4 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T18:47:00Z |
| publishDate | 2021 |
| publisher | Optical Society of America |
| record_format | dspace |
| spelling | oxford-uuid:0edac9fc-e3db-4734-a7a2-17b34213cdb42022-03-26T09:48:13Z3D super-resolution deep-tissue imaging in living miceJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:0edac9fc-e3db-4734-a7a2-17b34213cdb4EnglishSymplectic ElementsOptical Society of America2021Velasco, MGMZhang, MAntonello, JYuan, PAllgeyer, ESMay, DM'Saad, OKidd, PBarentine, AESGreco, VGrutzendler, JBooth, MJBewersdorf, JStimulated emission depletion (STED) microscopy enables the three-dimensional (3D) visualization of dynamic nanoscale structures in living cells, offering unique insights into their organization. However, 3D-STED imaging deep inside biological tissue is obstructed by optical aberrations and light scattering. We present a STED system that overcomes these challenges. Through the combination of two-photon excitation, adaptive optics, red-emitting organic dyes, and a long-working-distance water-immersion objective lens, our system achieves aberration-corrected 3D super-resolution imaging, which we demonstrate 164 µm deep in fixed mouse brain tissue and 76 µm deep in the brain of a living mouse. |
| spellingShingle | Velasco, MGM Zhang, M Antonello, J Yuan, P Allgeyer, ES May, D M'Saad, O Kidd, P Barentine, AES Greco, V Grutzendler, J Booth, MJ Bewersdorf, J 3D super-resolution deep-tissue imaging in living mice |
| title | 3D super-resolution deep-tissue imaging in living mice |
| title_full | 3D super-resolution deep-tissue imaging in living mice |
| title_fullStr | 3D super-resolution deep-tissue imaging in living mice |
| title_full_unstemmed | 3D super-resolution deep-tissue imaging in living mice |
| title_short | 3D super-resolution deep-tissue imaging in living mice |
| title_sort | 3d super resolution deep tissue imaging in living mice |
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