Spectral domain optical coherence tomography in mouse models of retinal degeneration.
PURPOSE: Spectral domain optical coherence tomography (SD-OCT) allows cross-sectional visualization of retinal structures in vivo. Here, the authors report the efficacy of a commercially available SD-OCT device to study mouse models of retinal degeneration. METHODS: C57BL/6 and BALB/c wild-type mic...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Journal article |
Language: | English |
Published: |
2009
|
_version_ | 1797053688541347840 |
---|---|
author | Huber, G Beck, S Grimm, C Sahaboglu-Tekgoz, A Paquet-Durand, F Wenzel, A Humphries, P Redmond, T Seeliger, M Fischer, M |
author_facet | Huber, G Beck, S Grimm, C Sahaboglu-Tekgoz, A Paquet-Durand, F Wenzel, A Humphries, P Redmond, T Seeliger, M Fischer, M |
author_sort | Huber, G |
collection | OXFORD |
description | PURPOSE: Spectral domain optical coherence tomography (SD-OCT) allows cross-sectional visualization of retinal structures in vivo. Here, the authors report the efficacy of a commercially available SD-OCT device to study mouse models of retinal degeneration. METHODS: C57BL/6 and BALB/c wild-type mice and three different mouse models of hereditary retinal degeneration (Rho(-/-), rd1, RPE65(-/-)) were investigated using confocal scanning laser ophthalmoscopy (cSLO) for en face visualization and SD-OCT for cross-sectional imaging of retinal structures. Histology was performed to correlate structural findings in SD-OCT with light microscopic data. RESULTS: In C57BL/6 and BALB/c mice, cSLO and SD-OCT imaging provided structural details of frequently used control animals (central retinal thickness, CRT(C57BL/6) = 237 +/- 2 microm and CRT(BALB/c) = 211 +/- 10 microm). RPE65(-/-) mice at 11 months of age showed a significant reduction of retinal thickness (CRT(RPE65) = 193 +/- 2 microm) with thinning of the outer nuclear layer. Rho(-/-) mice at P28 demonstrated degenerative changes mainly in the outer retinal layers (CRT(Rho) = 193 +/- 2 microm). Examining rd1 animals before and after the onset of retinal degeneration allowed monitoring of disease progression (CRT(rd1 P11) = 246 +/- 4 microm, CRT(rd1 P28) = 143 +/- 4 microm). Correlation of CRT assessed by histology and SD-OCT was high (r(2) = 0.897). CONCLUSIONS: The authors demonstrated cross-sectional visualization of retinal structures in wild-type mice and mouse models for retinal degeneration in vivo using a commercially available SD-OCT device. This method will help to reduce numbers of animals needed per study by allowing longitudinal study designs and will facilitate characterization of disease dynamics and evaluation of putative therapeutic effects after experimental interventions. |
first_indexed | 2024-03-06T18:47:11Z |
format | Journal article |
id | oxford-uuid:0eea888f-3db9-4be7-955c-38fcef0b33e4 |
institution | University of Oxford |
language | English |
last_indexed | 2024-03-06T18:47:11Z |
publishDate | 2009 |
record_format | dspace |
spelling | oxford-uuid:0eea888f-3db9-4be7-955c-38fcef0b33e42022-03-26T09:48:39ZSpectral domain optical coherence tomography in mouse models of retinal degeneration.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:0eea888f-3db9-4be7-955c-38fcef0b33e4EnglishSymplectic Elements at Oxford2009Huber, GBeck, SGrimm, CSahaboglu-Tekgoz, APaquet-Durand, FWenzel, AHumphries, PRedmond, TSeeliger, MFischer, M PURPOSE: Spectral domain optical coherence tomography (SD-OCT) allows cross-sectional visualization of retinal structures in vivo. Here, the authors report the efficacy of a commercially available SD-OCT device to study mouse models of retinal degeneration. METHODS: C57BL/6 and BALB/c wild-type mice and three different mouse models of hereditary retinal degeneration (Rho(-/-), rd1, RPE65(-/-)) were investigated using confocal scanning laser ophthalmoscopy (cSLO) for en face visualization and SD-OCT for cross-sectional imaging of retinal structures. Histology was performed to correlate structural findings in SD-OCT with light microscopic data. RESULTS: In C57BL/6 and BALB/c mice, cSLO and SD-OCT imaging provided structural details of frequently used control animals (central retinal thickness, CRT(C57BL/6) = 237 +/- 2 microm and CRT(BALB/c) = 211 +/- 10 microm). RPE65(-/-) mice at 11 months of age showed a significant reduction of retinal thickness (CRT(RPE65) = 193 +/- 2 microm) with thinning of the outer nuclear layer. Rho(-/-) mice at P28 demonstrated degenerative changes mainly in the outer retinal layers (CRT(Rho) = 193 +/- 2 microm). Examining rd1 animals before and after the onset of retinal degeneration allowed monitoring of disease progression (CRT(rd1 P11) = 246 +/- 4 microm, CRT(rd1 P28) = 143 +/- 4 microm). Correlation of CRT assessed by histology and SD-OCT was high (r(2) = 0.897). CONCLUSIONS: The authors demonstrated cross-sectional visualization of retinal structures in wild-type mice and mouse models for retinal degeneration in vivo using a commercially available SD-OCT device. This method will help to reduce numbers of animals needed per study by allowing longitudinal study designs and will facilitate characterization of disease dynamics and evaluation of putative therapeutic effects after experimental interventions. |
spellingShingle | Huber, G Beck, S Grimm, C Sahaboglu-Tekgoz, A Paquet-Durand, F Wenzel, A Humphries, P Redmond, T Seeliger, M Fischer, M Spectral domain optical coherence tomography in mouse models of retinal degeneration. |
title | Spectral domain optical coherence tomography in mouse models of retinal degeneration. |
title_full | Spectral domain optical coherence tomography in mouse models of retinal degeneration. |
title_fullStr | Spectral domain optical coherence tomography in mouse models of retinal degeneration. |
title_full_unstemmed | Spectral domain optical coherence tomography in mouse models of retinal degeneration. |
title_short | Spectral domain optical coherence tomography in mouse models of retinal degeneration. |
title_sort | spectral domain optical coherence tomography in mouse models of retinal degeneration |
work_keys_str_mv | AT huberg spectraldomainopticalcoherencetomographyinmousemodelsofretinaldegeneration AT becks spectraldomainopticalcoherencetomographyinmousemodelsofretinaldegeneration AT grimmc spectraldomainopticalcoherencetomographyinmousemodelsofretinaldegeneration AT sahaboglutekgoza spectraldomainopticalcoherencetomographyinmousemodelsofretinaldegeneration AT paquetdurandf spectraldomainopticalcoherencetomographyinmousemodelsofretinaldegeneration AT wenzela spectraldomainopticalcoherencetomographyinmousemodelsofretinaldegeneration AT humphriesp spectraldomainopticalcoherencetomographyinmousemodelsofretinaldegeneration AT redmondt spectraldomainopticalcoherencetomographyinmousemodelsofretinaldegeneration AT seeligerm spectraldomainopticalcoherencetomographyinmousemodelsofretinaldegeneration AT fischerm spectraldomainopticalcoherencetomographyinmousemodelsofretinaldegeneration |