Conservation of the function counts: homologous neurons express sequence-related neuropeptides that originate from different genes.
By means of single-cell matrix assisted laser desorption/ionization time-of-flight mass spectrometry, we analysed neuropeptide expression in all FXPRLamide/pheromone biosynthesis activating neuropeptide synthesizing neurons of the adult tobacco hawk moth, Manduca sexta. Mass spectra clearly suggest...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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2009
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| _version_ | 1826259276014813184 |
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| author | Neupert, S Huetteroth, W Schachtner, J Predel, R |
| author_facet | Neupert, S Huetteroth, W Schachtner, J Predel, R |
| author_sort | Neupert, S |
| collection | OXFORD |
| description | By means of single-cell matrix assisted laser desorption/ionization time-of-flight mass spectrometry, we analysed neuropeptide expression in all FXPRLamide/pheromone biosynthesis activating neuropeptide synthesizing neurons of the adult tobacco hawk moth, Manduca sexta. Mass spectra clearly suggest a completely identical processing of the pheromone biosynthesis activating neuropeptide-precursor in the mandibular, maxillary and labial neuromeres of the subesophageal ganglion. Only in the pban-neurons of the labial neuromere, products of two neuropeptide genes, namely the pban-gene and the capa-gene, were detected. Both of these genes expressed, amongst others, sequence-related neuropeptides (extended WFGPRLamides). We speculate that the expression of the two neuropeptide genes is a plesiomorph character typical of moths. A detailed examination of the neuroanatomy and the peptidome of the (two) pban-neurons in the labial neuromere of moths with homologous neurons of different insects indicates a strong conservation of the function of this neuroendocrine system. In other insects, however, the labial neurons either express products of the fxprl-gene or products of the capa-gene. The processing of the respective genes is reduced to extended WFGPRLamides in each case and yields a unique peptidome in the labial cells. Thus, sequence-related messenger molecules are always produced in these cells and it seems that the respective neurons recruited different neuropeptide genes for this motif. |
| first_indexed | 2024-03-06T18:47:18Z |
| format | Journal article |
| id | oxford-uuid:0ef2053f-0e1f-4316-bcbb-a0cae27161b4 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T18:47:18Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | oxford-uuid:0ef2053f-0e1f-4316-bcbb-a0cae27161b42022-03-26T09:48:48ZConservation of the function counts: homologous neurons express sequence-related neuropeptides that originate from different genes.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:0ef2053f-0e1f-4316-bcbb-a0cae27161b4EnglishSymplectic Elements at Oxford2009Neupert, SHuetteroth, WSchachtner, JPredel, RBy means of single-cell matrix assisted laser desorption/ionization time-of-flight mass spectrometry, we analysed neuropeptide expression in all FXPRLamide/pheromone biosynthesis activating neuropeptide synthesizing neurons of the adult tobacco hawk moth, Manduca sexta. Mass spectra clearly suggest a completely identical processing of the pheromone biosynthesis activating neuropeptide-precursor in the mandibular, maxillary and labial neuromeres of the subesophageal ganglion. Only in the pban-neurons of the labial neuromere, products of two neuropeptide genes, namely the pban-gene and the capa-gene, were detected. Both of these genes expressed, amongst others, sequence-related neuropeptides (extended WFGPRLamides). We speculate that the expression of the two neuropeptide genes is a plesiomorph character typical of moths. A detailed examination of the neuroanatomy and the peptidome of the (two) pban-neurons in the labial neuromere of moths with homologous neurons of different insects indicates a strong conservation of the function of this neuroendocrine system. In other insects, however, the labial neurons either express products of the fxprl-gene or products of the capa-gene. The processing of the respective genes is reduced to extended WFGPRLamides in each case and yields a unique peptidome in the labial cells. Thus, sequence-related messenger molecules are always produced in these cells and it seems that the respective neurons recruited different neuropeptide genes for this motif. |
| spellingShingle | Neupert, S Huetteroth, W Schachtner, J Predel, R Conservation of the function counts: homologous neurons express sequence-related neuropeptides that originate from different genes. |
| title | Conservation of the function counts: homologous neurons express sequence-related neuropeptides that originate from different genes. |
| title_full | Conservation of the function counts: homologous neurons express sequence-related neuropeptides that originate from different genes. |
| title_fullStr | Conservation of the function counts: homologous neurons express sequence-related neuropeptides that originate from different genes. |
| title_full_unstemmed | Conservation of the function counts: homologous neurons express sequence-related neuropeptides that originate from different genes. |
| title_short | Conservation of the function counts: homologous neurons express sequence-related neuropeptides that originate from different genes. |
| title_sort | conservation of the function counts homologous neurons express sequence related neuropeptides that originate from different genes |
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