Anti-tumor DNA vaccines based on the expression of human papillomavirus-16 E6/E7 oncoproteins genetically fused with the glycoprotein D from herpes simplex virus-1.
DNA vaccines encoding the human papillomavirus type-16 (HPV-16) E6 and E7 oncoproteins genetically fused to the human herpes simplex virus type 1 (HSV-1) gD protein were tested in mice for induction of T cell-mediated immunity and protection against tumor cell challenge. Hybrid genes, generated afte...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
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2005
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| _version_ | 1797053791075303424 |
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| author | Lasaro, M Diniz, M Reyes-Sandoval, A Ertl, H Ferreira, L |
| author_facet | Lasaro, M Diniz, M Reyes-Sandoval, A Ertl, H Ferreira, L |
| author_sort | Lasaro, M |
| collection | OXFORD |
| description | DNA vaccines encoding the human papillomavirus type-16 (HPV-16) E6 and E7 oncoproteins genetically fused to the human herpes simplex virus type 1 (HSV-1) gD protein were tested in mice for induction of T cell-mediated immunity and protection against tumor cell challenge. Hybrid genes, generated after insertion of E6 or E7-encoding sequences into internal sites of the gD-encoding gene, were transcribed in vitro and the chimeric proteins were expressed at the surface of in vitro-transfected mammalian cells. Female C57BL/6 mice immunized with 4 intramuscular doses (100 microg of DNA/dose) of the DNA vaccines encoding E7 efficiently generated E7-specific CD8(+) T cells. Vaccination of mice with the DNA vaccines encoding the E7, or both E6 and E7, conferred complete protection to challenges from TC-1 tumor cells and partial therapeutic effect (40%) in mice inoculated with TC-1 cells on the same day or 5 days prior to the first vaccine dose. |
| first_indexed | 2024-03-06T18:48:30Z |
| format | Journal article |
| id | oxford-uuid:0f5d695a-9806-4b16-8da1-15bead08f48f |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T18:48:30Z |
| publishDate | 2005 |
| record_format | dspace |
| spelling | oxford-uuid:0f5d695a-9806-4b16-8da1-15bead08f48f2022-03-26T09:50:54ZAnti-tumor DNA vaccines based on the expression of human papillomavirus-16 E6/E7 oncoproteins genetically fused with the glycoprotein D from herpes simplex virus-1.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:0f5d695a-9806-4b16-8da1-15bead08f48fEnglishSymplectic Elements at Oxford2005Lasaro, MDiniz, MReyes-Sandoval, AErtl, HFerreira, LDNA vaccines encoding the human papillomavirus type-16 (HPV-16) E6 and E7 oncoproteins genetically fused to the human herpes simplex virus type 1 (HSV-1) gD protein were tested in mice for induction of T cell-mediated immunity and protection against tumor cell challenge. Hybrid genes, generated after insertion of E6 or E7-encoding sequences into internal sites of the gD-encoding gene, were transcribed in vitro and the chimeric proteins were expressed at the surface of in vitro-transfected mammalian cells. Female C57BL/6 mice immunized with 4 intramuscular doses (100 microg of DNA/dose) of the DNA vaccines encoding E7 efficiently generated E7-specific CD8(+) T cells. Vaccination of mice with the DNA vaccines encoding the E7, or both E6 and E7, conferred complete protection to challenges from TC-1 tumor cells and partial therapeutic effect (40%) in mice inoculated with TC-1 cells on the same day or 5 days prior to the first vaccine dose. |
| spellingShingle | Lasaro, M Diniz, M Reyes-Sandoval, A Ertl, H Ferreira, L Anti-tumor DNA vaccines based on the expression of human papillomavirus-16 E6/E7 oncoproteins genetically fused with the glycoprotein D from herpes simplex virus-1. |
| title | Anti-tumor DNA vaccines based on the expression of human papillomavirus-16 E6/E7 oncoproteins genetically fused with the glycoprotein D from herpes simplex virus-1. |
| title_full | Anti-tumor DNA vaccines based on the expression of human papillomavirus-16 E6/E7 oncoproteins genetically fused with the glycoprotein D from herpes simplex virus-1. |
| title_fullStr | Anti-tumor DNA vaccines based on the expression of human papillomavirus-16 E6/E7 oncoproteins genetically fused with the glycoprotein D from herpes simplex virus-1. |
| title_full_unstemmed | Anti-tumor DNA vaccines based on the expression of human papillomavirus-16 E6/E7 oncoproteins genetically fused with the glycoprotein D from herpes simplex virus-1. |
| title_short | Anti-tumor DNA vaccines based on the expression of human papillomavirus-16 E6/E7 oncoproteins genetically fused with the glycoprotein D from herpes simplex virus-1. |
| title_sort | anti tumor dna vaccines based on the expression of human papillomavirus 16 e6 e7 oncoproteins genetically fused with the glycoprotein d from herpes simplex virus 1 |
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