The palisade layer of the poxvirus core is composed of flexible A10 trimers
Due to its asymmetric shape, size and compactness, the structure of the infectious mature virus (MV) of vaccinia virus (VACV), the best-studied poxvirus, remains poorly understood. Instead, subviral particles, in particular membrane-free viral cores, have been studied with cryo-electron microscopy....
| Egile Nagusiak: | , , , , , , , , , , , , |
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| Formatua: | Journal article |
| Hizkuntza: | English |
| Argitaratua: |
Nature Research
2024
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| _version_ | 1826314791984037888 |
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| author | Liu, J Corroyer-Dulmont, S Pražák, V Khusainov, I Bahrami, K Welsch, S Vasishtan, D Obarska-Kosińska, A Thorkelsson, SR Grünewald, K Quemin, ERJ Turoňová, B Locker, JK |
| author_facet | Liu, J Corroyer-Dulmont, S Pražák, V Khusainov, I Bahrami, K Welsch, S Vasishtan, D Obarska-Kosińska, A Thorkelsson, SR Grünewald, K Quemin, ERJ Turoňová, B Locker, JK |
| author_sort | Liu, J |
| collection | OXFORD |
| description | Due to its asymmetric shape, size and compactness, the structure of the infectious mature virus (MV) of vaccinia virus (VACV), the best-studied poxvirus, remains poorly understood. Instead, subviral particles, in particular membrane-free viral cores, have been studied with cryo-electron microscopy. Here, we compared viral cores obtained by detergent stripping of MVs with cores in the cellular cytoplasm, early in infection. We focused on the prominent palisade layer on the core surface, combining cryo-electron tomography, subtomogram averaging and AlphaFold2 structure prediction. We showed that the palisade is composed of densely packed trimers of the major core protein A10. Trimers display a random order and their classification indicates structural flexibility. A10 on cytoplasmic cores is organized in a similar manner, indicating that the structures obtained in vitro are physiologically relevant. We discuss our results in the context of the VACV replicative cycle, and the assembly and disassembly of the infectious MV. |
| first_indexed | 2024-09-25T04:14:53Z |
| format | Journal article |
| id | oxford-uuid:0f9b15e5-b723-4276-bca1-87c69fc88079 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-12-09T03:13:22Z |
| publishDate | 2024 |
| publisher | Nature Research |
| record_format | dspace |
| spelling | oxford-uuid:0f9b15e5-b723-4276-bca1-87c69fc880792024-10-16T09:16:04ZThe palisade layer of the poxvirus core is composed of flexible A10 trimersJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:0f9b15e5-b723-4276-bca1-87c69fc88079EnglishJisc Publications RouterNature Research2024Liu, JCorroyer-Dulmont, SPražák, VKhusainov, IBahrami, KWelsch, SVasishtan, DObarska-Kosińska, AThorkelsson, SRGrünewald, KQuemin, ERJTuroňová, BLocker, JKDue to its asymmetric shape, size and compactness, the structure of the infectious mature virus (MV) of vaccinia virus (VACV), the best-studied poxvirus, remains poorly understood. Instead, subviral particles, in particular membrane-free viral cores, have been studied with cryo-electron microscopy. Here, we compared viral cores obtained by detergent stripping of MVs with cores in the cellular cytoplasm, early in infection. We focused on the prominent palisade layer on the core surface, combining cryo-electron tomography, subtomogram averaging and AlphaFold2 structure prediction. We showed that the palisade is composed of densely packed trimers of the major core protein A10. Trimers display a random order and their classification indicates structural flexibility. A10 on cytoplasmic cores is organized in a similar manner, indicating that the structures obtained in vitro are physiologically relevant. We discuss our results in the context of the VACV replicative cycle, and the assembly and disassembly of the infectious MV. |
| spellingShingle | Liu, J Corroyer-Dulmont, S Pražák, V Khusainov, I Bahrami, K Welsch, S Vasishtan, D Obarska-Kosińska, A Thorkelsson, SR Grünewald, K Quemin, ERJ Turoňová, B Locker, JK The palisade layer of the poxvirus core is composed of flexible A10 trimers |
| title | The palisade layer of the poxvirus core is composed of flexible A10 trimers |
| title_full | The palisade layer of the poxvirus core is composed of flexible A10 trimers |
| title_fullStr | The palisade layer of the poxvirus core is composed of flexible A10 trimers |
| title_full_unstemmed | The palisade layer of the poxvirus core is composed of flexible A10 trimers |
| title_short | The palisade layer of the poxvirus core is composed of flexible A10 trimers |
| title_sort | palisade layer of the poxvirus core is composed of flexible a10 trimers |
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