DNA triple helix stabilisation by covalent attachment of a triplex-specific ligand.
We have prepared oligonucleotides with a naphthylquinoline triplex-binding ligand covalently tethered to the 5'-end and have used UV-melting and DNase I footprinting to examine the stability of intra- and inter-molecular triplexes containing this modification. We find that covalent attachment o...
| Автори: | , , , , , |
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| Формат: | Journal article |
| Мова: | English |
| Опубліковано: |
1999
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| _version_ | 1826259401563963392 |
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| author | Keppler, MD McKeen, C Zegrocka, O Strekowski, L Brown, T Fox, K |
| author_facet | Keppler, MD McKeen, C Zegrocka, O Strekowski, L Brown, T Fox, K |
| author_sort | Keppler, MD |
| collection | OXFORD |
| description | We have prepared oligonucleotides with a naphthylquinoline triplex-binding ligand covalently tethered to the 5'-end and have used UV-melting and DNase I footprinting to examine the stability of intra- and inter-molecular triplexes containing this modification. We find that covalent attachment of the ligand increases the melting temperature of intramolecular 6-mer triplexes by about 14 K, and increases the binding of 9-mer oligonucleotides to their duplex target sites by about 60-fold. |
| first_indexed | 2024-03-06T18:49:17Z |
| format | Journal article |
| id | oxford-uuid:0fa28284-70b8-48ae-a084-58f635cbf577 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T18:49:17Z |
| publishDate | 1999 |
| record_format | dspace |
| spelling | oxford-uuid:0fa28284-70b8-48ae-a084-58f635cbf5772022-03-26T09:52:11ZDNA triple helix stabilisation by covalent attachment of a triplex-specific ligand.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:0fa28284-70b8-48ae-a084-58f635cbf577EnglishSymplectic Elements at Oxford1999Keppler, MDMcKeen, CZegrocka, OStrekowski, LBrown, TFox, KWe have prepared oligonucleotides with a naphthylquinoline triplex-binding ligand covalently tethered to the 5'-end and have used UV-melting and DNase I footprinting to examine the stability of intra- and inter-molecular triplexes containing this modification. We find that covalent attachment of the ligand increases the melting temperature of intramolecular 6-mer triplexes by about 14 K, and increases the binding of 9-mer oligonucleotides to their duplex target sites by about 60-fold. |
| spellingShingle | Keppler, MD McKeen, C Zegrocka, O Strekowski, L Brown, T Fox, K DNA triple helix stabilisation by covalent attachment of a triplex-specific ligand. |
| title | DNA triple helix stabilisation by covalent attachment of a triplex-specific ligand. |
| title_full | DNA triple helix stabilisation by covalent attachment of a triplex-specific ligand. |
| title_fullStr | DNA triple helix stabilisation by covalent attachment of a triplex-specific ligand. |
| title_full_unstemmed | DNA triple helix stabilisation by covalent attachment of a triplex-specific ligand. |
| title_short | DNA triple helix stabilisation by covalent attachment of a triplex-specific ligand. |
| title_sort | dna triple helix stabilisation by covalent attachment of a triplex specific ligand |
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