Detergent-free mass spectrometry of membrane protein complexes

We developed a method that allows release of intact membrane protein complexes from amphipols, bicelles and nanodiscs in the gas phase for observation by mass spectrometry (MS). Current methods involve release of membrane protein complexes from detergent micelles, which reveals subunit composition a...

Ausführliche Beschreibung

Bibliographische Detailangaben
Hauptverfasser: Hopper, J, Yu, Y, Li, D, Raymond, A, Bostock, M, Liko, I, Mikhailov, V, Laganowsky, A, Benesch, J, Caffrey, M, Nietlispach, D, Robinson, C
Format: Journal article
Sprache:English
Veröffentlicht: 2013