Human immunodeficiency virus type 1 virological synapse formation in T cells requires lipid raft integrity.
Human immunodeficiency virus type 1 (HIV-1) can spread directly between T cells by forming a supramolecular structure termed a virological synapse (VS). HIV-1 envelope glycoproteins (Env) are required for VS assembly, but their mode of recruitment is unclear. We investigated the distribution of GM1-...
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| Format: | Journal article |
| Language: | English |
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2005
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| _version_ | 1826259459799777280 |
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| author | Jolly, C Sattentau, Q |
| author_facet | Jolly, C Sattentau, Q |
| author_sort | Jolly, C |
| collection | OXFORD |
| description | Human immunodeficiency virus type 1 (HIV-1) can spread directly between T cells by forming a supramolecular structure termed a virological synapse (VS). HIV-1 envelope glycoproteins (Env) are required for VS assembly, but their mode of recruitment is unclear. We investigated the distribution of GM1-rich lipid rafts in HIV-1-infected (effector) T cells and observed Env colocalization with polarized raft markers GM1 and CD59 but not with the transferrin receptor that is excluded from lipid rafts. In conjugates of effector T cells and target CD4+ T cells, GM1, Env, and Gag relocated to the cell-cell interface. The depletion of cholesterol in the infected cell dispersed Env and GM1 within the plasma membrane, eliminated Gag clustering at the site of cell-cell contact, and abolished assembly of the VS. Raft integrity is therefore critical for Env and Gag co-clustering and VS assembly in T-cell conjugates. |
| first_indexed | 2024-03-06T18:50:14Z |
| format | Journal article |
| id | oxford-uuid:0ff938a5-e792-471f-8aed-7e6ab8a7a2c7 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T18:50:14Z |
| publishDate | 2005 |
| record_format | dspace |
| spelling | oxford-uuid:0ff938a5-e792-471f-8aed-7e6ab8a7a2c72022-03-26T09:53:58ZHuman immunodeficiency virus type 1 virological synapse formation in T cells requires lipid raft integrity.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:0ff938a5-e792-471f-8aed-7e6ab8a7a2c7EnglishSymplectic Elements at Oxford2005Jolly, CSattentau, QHuman immunodeficiency virus type 1 (HIV-1) can spread directly between T cells by forming a supramolecular structure termed a virological synapse (VS). HIV-1 envelope glycoproteins (Env) are required for VS assembly, but their mode of recruitment is unclear. We investigated the distribution of GM1-rich lipid rafts in HIV-1-infected (effector) T cells and observed Env colocalization with polarized raft markers GM1 and CD59 but not with the transferrin receptor that is excluded from lipid rafts. In conjugates of effector T cells and target CD4+ T cells, GM1, Env, and Gag relocated to the cell-cell interface. The depletion of cholesterol in the infected cell dispersed Env and GM1 within the plasma membrane, eliminated Gag clustering at the site of cell-cell contact, and abolished assembly of the VS. Raft integrity is therefore critical for Env and Gag co-clustering and VS assembly in T-cell conjugates. |
| spellingShingle | Jolly, C Sattentau, Q Human immunodeficiency virus type 1 virological synapse formation in T cells requires lipid raft integrity. |
| title | Human immunodeficiency virus type 1 virological synapse formation in T cells requires lipid raft integrity. |
| title_full | Human immunodeficiency virus type 1 virological synapse formation in T cells requires lipid raft integrity. |
| title_fullStr | Human immunodeficiency virus type 1 virological synapse formation in T cells requires lipid raft integrity. |
| title_full_unstemmed | Human immunodeficiency virus type 1 virological synapse formation in T cells requires lipid raft integrity. |
| title_short | Human immunodeficiency virus type 1 virological synapse formation in T cells requires lipid raft integrity. |
| title_sort | human immunodeficiency virus type 1 virological synapse formation in t cells requires lipid raft integrity |
| work_keys_str_mv | AT jollyc humanimmunodeficiencyvirustype1virologicalsynapseformationintcellsrequireslipidraftintegrity AT sattentauq humanimmunodeficiencyvirustype1virologicalsynapseformationintcellsrequireslipidraftintegrity |