Ultrasensitive amplicon barcoding for next-generation sequencing facilitating sequence error and amplification-bias correction
The ability to accurately characterize DNA variant proportions using PCR amplification is key to many genetic studies, including studying tumor heterogeneity, 16S microbiome, viral and immune receptor sequencing. We develop a novel generalizable ultrasensitive amplicon barcoding approach that signif...
| Main Authors: | , , , , |
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| Format: | Journal article |
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Nature Research
2020
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| _version_ | 1797053938796593152 |
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| author | Ahmed, I Tucci, FA Aflalo, A Smith, KGC Bashford-Rogers, R |
| author_facet | Ahmed, I Tucci, FA Aflalo, A Smith, KGC Bashford-Rogers, R |
| author_sort | Ahmed, I |
| collection | OXFORD |
| description | The ability to accurately characterize DNA variant proportions using PCR amplification is key to many genetic studies, including studying tumor heterogeneity, 16S microbiome, viral and immune receptor sequencing. We develop a novel generalizable ultrasensitive amplicon barcoding approach that significantly reduces the inflation/deflation of DNA variant proportions due to PCR amplification biases and sequencing errors. This method was applied to immune receptor sequencing, where it significantly improves the quality and estimation of diversity of the resulting library. |
| first_indexed | 2024-03-06T18:50:35Z |
| format | Journal article |
| id | oxford-uuid:10158912-2fbc-4af4-bdad-74300d7e7b5f |
| institution | University of Oxford |
| last_indexed | 2024-03-06T18:50:35Z |
| publishDate | 2020 |
| publisher | Nature Research |
| record_format | dspace |
| spelling | oxford-uuid:10158912-2fbc-4af4-bdad-74300d7e7b5f2022-03-26T09:54:34ZUltrasensitive amplicon barcoding for next-generation sequencing facilitating sequence error and amplification-bias correctionJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:10158912-2fbc-4af4-bdad-74300d7e7b5fSymplectic ElementsNature Research 2020Ahmed, ITucci, FAAflalo, ASmith, KGCBashford-Rogers, RThe ability to accurately characterize DNA variant proportions using PCR amplification is key to many genetic studies, including studying tumor heterogeneity, 16S microbiome, viral and immune receptor sequencing. We develop a novel generalizable ultrasensitive amplicon barcoding approach that significantly reduces the inflation/deflation of DNA variant proportions due to PCR amplification biases and sequencing errors. This method was applied to immune receptor sequencing, where it significantly improves the quality and estimation of diversity of the resulting library. |
| spellingShingle | Ahmed, I Tucci, FA Aflalo, A Smith, KGC Bashford-Rogers, R Ultrasensitive amplicon barcoding for next-generation sequencing facilitating sequence error and amplification-bias correction |
| title | Ultrasensitive amplicon barcoding for next-generation sequencing facilitating sequence error and amplification-bias correction |
| title_full | Ultrasensitive amplicon barcoding for next-generation sequencing facilitating sequence error and amplification-bias correction |
| title_fullStr | Ultrasensitive amplicon barcoding for next-generation sequencing facilitating sequence error and amplification-bias correction |
| title_full_unstemmed | Ultrasensitive amplicon barcoding for next-generation sequencing facilitating sequence error and amplification-bias correction |
| title_short | Ultrasensitive amplicon barcoding for next-generation sequencing facilitating sequence error and amplification-bias correction |
| title_sort | ultrasensitive amplicon barcoding for next generation sequencing facilitating sequence error and amplification bias correction |
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