Detection of CFTR transgene mRNA expression in respiratory epithelium isolated from the murine nasal cavity.

Detection of CFTR transgene mRNA expression in respiratory epithelium isolated from the murine nasal cavity.

BACKGROUND: When assessing the efficacy of gene transfer agents (GTAs) for cystic fibrosis (CF) gene therapy, we routinely evaluate gene transfer in the mouse nose and measure transfection efficiency by assessing transgene-specific mRNA using the real-time (TaqMan) quantitative reverse transcriptase...

وصف كامل

التفاصيل البيبلوغرافية
المؤلفون الرئيسيون:	Holder, E, Stevenson, B, Farley, R, Hilliard, T, Wodehouse, T, Somerton, L, Larsen, M, O'Donoghue, J, Coles, R, Scheule, R, Cheng, S, Gill, DR, Hyde, S, Griesenbach, U, Alton, E, Porteous, D, Boyd, A
التنسيق:	Journal article
اللغة:	English
منشور في:	2010

مواد مشابهة

Assessment of CFTR function after gene transfer in vitro and in vivo.
حسب: Griesenbach, U, وآخرون
منشور في: (2008)

SAFETY AND EXPRESSION OF A SINGLE DOSE OF LIPID-MEDIATED CFTR GENE THERAPY TO THE UPPER AND LOWER AIRWAYS OF PATIENTS WITH CF
حسب: Davies, J, وآخرون
منشور في: (2011)

SAFETY AND EXPRESSION OF A SINGLE DOSE OF LIPID-MEDIATED CFTR GENE THERAPY TO THE UPPER AND LOWER AIRWAYS OF PATIENTS WITH CYSTIC FIBROSIS
حسب: Davies, G, وآخرون
منشور في: (2011)

Sendai virus-mediated CFTR gene transfer to the airway epithelium.
حسب: Ferrari, S, وآخرون
منشور في: (2007)

Self-reactive CFTR T cells in humans: implications for gene therapy.
حسب: Calcedo, R, وآخرون
منشور في: (2013)

REPEAT ADMINISTRATION OF GL67A/PGM169 IS FEASIBLE, SAFE, AND PRODUCES ENDOGENOUS LEVELS OF CFTR EXPRESSION AFTER 12 DOSES
حسب: Alton, E, وآخرون
منشور في: (2012)

Cumulative CFTR expression following repeated aerosol delivery of non-viral pGM169/GL67A formulation to mouse lung
حسب: Alton, E, وآخرون
منشور في: (2012)

Development of an immunocytochemical assay to detect human CFTR expression following gene therapy
حسب: Davidson, H, وآخرون
منشور في: (2008)

An immunocytochemical assay to detect human CFTR expression following gene transfer.
حسب: Davidson, H, وآخرون
منشور في: (2009)

LacZ siRNA and antisense DNA decrease beta-galactosidase mRNA but not protein expression in the airways of K18-lacZ transgenic mice
حسب: Griesenbach, U, وآخرون
منشور في: (2004)

Apical CFTR expression in human nasal epithelium correlates with lung disease in cystic fibrosis.
حسب: Marit Arianne van Meegen, وآخرون
منشور في: (2013-01-01)

Strategies for long-term expression of transgenes in the respiratory epithelium.
حسب: Gill, DR, وآخرون
منشور في: (2010)

CFTR, <it>P. aeruginosa</it> and apoptosis in respiratory epithelium
حسب: Burrows Lori, وآخرون
منشور في: (2000-10-01)

A PHASE 2B DOUBLE-BLIND, PLACEBO-CONTROLLED TRIAL OF NON-VIRAL MEDIATED GENE THERAPY FOR CF
حسب: Alton, E, وآخرون
منشور في: (2013)

A randomized, double-blind, placebo-controlled trial of repeated nebulisation of non-viral CFTR gene therapy in patients with cystic fibrosis
حسب: Alton, E, وآخرون
منشور في: (2015)

TOXICOLOGY STUDIES IN SUPPORT OF THE UK CF GENE THERAPY CONSORTIUM'S MULTI-DOSE CLINICAL TRIAL
حسب: Griesenbach, U, وآخرون
منشور في: (2011)

Characterization of nasal potential difference in cftr knockout and F508del-CFTR mice.
حسب: Emilie Lyne Saussereau, وآخرون
منشور في: (2013-01-01)

Adenovirus-mediated in utero expression of CFTR does not improve survival of CFTR knockout mice.
حسب: Davies, L, وآخرون
منشور في: (2008)

Down-regulation of the epithelial sodium channel ENaC through antisense oligonucleotides
حسب: Griesenbach, U, وآخرون
منشور في: (2004)

Assessment of the air-liquid interface culture model as a tool to validate efficacy of a rSIV.F/HN-CFTR vector
حسب: Sergijenko, A, وآخرون
منشور في: (2021)

Putting bicarbonate on the spot: pharmacological insights for CFTR correction in the airway epithelium
حسب: Miroslaw Zajac, وآخرون
منشور في: (2023-12-01)

Nonspecific binding of common anti-CFTR antibodies in ciliated cells of human airway epithelium
حسب: Yukiko Sato, وآخرون
منشور في: (2021-12-01)

Pro-inflammatory cytokines stimulate CFTR-dependent anion secretion in pancreatic ductal epithelium
حسب: Dora Angyal, وآخرون
منشور في: (2024-01-01)

Low levels of CFTR gene transfer with F/HN pseudotyped lentivirus are sufficient to generate ion transport correction in airway cell cultures from cystic fibrosis patients
حسب: Sergijenko, A, وآخرون
منشور في: (2020)

Toxicology study assessing efficacy and safety of repeated administration of lipid/DNA complexes to mouse lung.
حسب: Alton, E, وآخرون
منشور في: (2014)

Toxicology study assessing efficacy and safety of repeated administration of lipid/DNA complexes to mouse lung
حسب: Alton, E, وآخرون
منشور في: (2014)

Optimizing nasal potential difference analysis for CFTR modulator development: assessment of ivacaftor in CF subjects with the G551D-CFTR mutation.
حسب: Steven M Rowe, وآخرون
منشور في: (2013-01-01)

Inefficient cationic lipid-mediated siRNA and antisense oligonucleotide transfer to airway epithelial cells in vivo.
حسب: Griesenbach, U, وآخرون
منشور في: (2006)

Limitations of the murine nose in the development of nonviral airway gene transfer.
حسب: Griesenbach, U, وآخرون
منشور في: (2010)

DEVELOPMENT, PRODUCTION AND EVALUATION OF CLINICAL GRADE CFTR EXPRESSION PLASMID FOR CF LUNG GENE THERAPY
حسب: Gill, DR, وآخرون
منشور في: (2010)

Optimizing aerosol gene delivery and expression in the ovine lung.
حسب: McLachlan, G, وآخرون
منشور في: (2007)

Intestinal current measurement detects age-dependent differences in CFTR function in rectal epithelium
حسب: Simon Y. Graeber, وآخرون
منشور في: (2025-02-01)

Is CFTR-delF508 really absent from the apical membrane of the airway epithelium?
حسب: Lee A Borthwick, وآخرون
منشور في: (2011-01-01)

Novel vector improves efficiency of gene transfer to the airway epithelium
حسب: Pickles Raymond, وآخرون
منشور في: (2000-12-01)

The use of carboxymethylcellulose gel to increase non-viral gene transfer in mouse airways.
حسب: Griesenbach, U, وآخرون
منشور في: (2010)

CFTR expression analysis in human nasal epithelial cells by flow cytometry.
حسب: Marit A van Meegen, وآخرون
منشور في: (2011-01-01)

Correction of CFTR function in nasal epithelial cells from cystic fibrosis patients predicts improvement of respiratory function by CFTR modulators
حسب: Iwona M. Pranke, وآخرون
منشور في: (2017-08-01)

LONGITUDINAL ASSESSMENT OF BIOMARKERS FOR CLINICAL TRIALS OF NOVEL THERAPEUTIC AGENTS: THE RUN-IN STUDY
حسب: Alton, E, وآخرون
منشور في: (2010)

Non-viral CFTR gene delivery to the lungs of Cystic Fibrosis patients
حسب: Hyde, S
منشور في: (2013)

A randomised, double-blind, placebo-controlled phase IIB clinical trial of repeated application of gene therapy in patients with cystic fibrosis
حسب: Alton, E, وآخرون
منشور في: (2013)