Detection of CFTR transgene mRNA expression in respiratory epithelium isolated from the murine nasal cavity.

Detection of CFTR transgene mRNA expression in respiratory epithelium isolated from the murine nasal cavity.

BACKGROUND: When assessing the efficacy of gene transfer agents (GTAs) for cystic fibrosis (CF) gene therapy, we routinely evaluate gene transfer in the mouse nose and measure transfection efficiency by assessing transgene-specific mRNA using the real-time (TaqMan) quantitative reverse transcriptase...

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Những tác giả chính:	Holder, E, Stevenson, B, Farley, R, Hilliard, T, Wodehouse, T, Somerton, L, Larsen, M, O'Donoghue, J, Coles, R, Scheule, R, Cheng, S, Gill, DR, Hyde, S, Griesenbach, U, Alton, E, Porteous, D, Boyd, A
Định dạng:	Journal article
Ngôn ngữ:	English
Được phát hành:	2010

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