Chromatin condensation fluctuations rather than steady-state predict chromatin accessibility
Chromatin accessibility to protein factors is critical for genome activities. However, the dynamic properties of chromatin higher-order structures that regulate its accessibility are poorly understood. Here, we took advantage of the microenvironment sensitivity of the fluorescence lifetime of EGFP-H...
| Main Authors: | , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
Oxford University Press
2019
|
| _version_ | 1797054044344156160 |
|---|---|
| author | Audugé, N Padilla-Parra, S Tramier, M Borghi, N Coppey-Moisan, M |
| author_facet | Audugé, N Padilla-Parra, S Tramier, M Borghi, N Coppey-Moisan, M |
| author_sort | Audugé, N |
| collection | OXFORD |
| description | Chromatin accessibility to protein factors is critical for genome activities. However, the dynamic properties of chromatin higher-order structures that regulate its accessibility are poorly understood. Here, we took advantage of the microenvironment sensitivity of the fluorescence lifetime of EGFP-H4 histone incorporated in chromatin to map in the nucleus of live cells the dynamics of chromatin condensation and its direct interaction with a tail acetylation recognition domain (the double bromodomain module of human TAFII250, dBD). We reveal chromatin condensation fluctuations supported by mechanisms fundamentally distinct from that of condensation. Fluctuations are spontaneous, yet their amplitudes are affected by their sub-nuclear localization and by distinct and competing mechanisms dependent on histone acetylation, ATP and both. Moreover, we show that accessibility of acetylated histone H4 to dBD is not restricted by chromatin condensation nor predicted by acetylation, rather, it is predicted by chromatin condensation fluctuations. |
| first_indexed | 2024-03-06T18:52:01Z |
| format | Journal article |
| id | oxford-uuid:108e30e4-6d7b-449e-9e32-b1cb1b7b8df4 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T18:52:01Z |
| publishDate | 2019 |
| publisher | Oxford University Press |
| record_format | dspace |
| spelling | oxford-uuid:108e30e4-6d7b-449e-9e32-b1cb1b7b8df42022-03-26T09:57:08ZChromatin condensation fluctuations rather than steady-state predict chromatin accessibilityJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:108e30e4-6d7b-449e-9e32-b1cb1b7b8df4EnglishSymplectic Elements at OxfordOxford University Press2019Audugé, NPadilla-Parra, STramier, MBorghi, NCoppey-Moisan, MChromatin accessibility to protein factors is critical for genome activities. However, the dynamic properties of chromatin higher-order structures that regulate its accessibility are poorly understood. Here, we took advantage of the microenvironment sensitivity of the fluorescence lifetime of EGFP-H4 histone incorporated in chromatin to map in the nucleus of live cells the dynamics of chromatin condensation and its direct interaction with a tail acetylation recognition domain (the double bromodomain module of human TAFII250, dBD). We reveal chromatin condensation fluctuations supported by mechanisms fundamentally distinct from that of condensation. Fluctuations are spontaneous, yet their amplitudes are affected by their sub-nuclear localization and by distinct and competing mechanisms dependent on histone acetylation, ATP and both. Moreover, we show that accessibility of acetylated histone H4 to dBD is not restricted by chromatin condensation nor predicted by acetylation, rather, it is predicted by chromatin condensation fluctuations. |
| spellingShingle | Audugé, N Padilla-Parra, S Tramier, M Borghi, N Coppey-Moisan, M Chromatin condensation fluctuations rather than steady-state predict chromatin accessibility |
| title | Chromatin condensation fluctuations rather than steady-state predict chromatin accessibility |
| title_full | Chromatin condensation fluctuations rather than steady-state predict chromatin accessibility |
| title_fullStr | Chromatin condensation fluctuations rather than steady-state predict chromatin accessibility |
| title_full_unstemmed | Chromatin condensation fluctuations rather than steady-state predict chromatin accessibility |
| title_short | Chromatin condensation fluctuations rather than steady-state predict chromatin accessibility |
| title_sort | chromatin condensation fluctuations rather than steady state predict chromatin accessibility |
| work_keys_str_mv | AT audugen chromatincondensationfluctuationsratherthansteadystatepredictchromatinaccessibility AT padillaparras chromatincondensationfluctuationsratherthansteadystatepredictchromatinaccessibility AT tramierm chromatincondensationfluctuationsratherthansteadystatepredictchromatinaccessibility AT borghin chromatincondensationfluctuationsratherthansteadystatepredictchromatinaccessibility AT coppeymoisanm chromatincondensationfluctuationsratherthansteadystatepredictchromatinaccessibility |