A method to quantify binding of unlabeled peptides to class I MHC molecules and detect their allele specificity.
A general method has been developed for measuring the stabilization of class I MHC molecules in extracts of the mutant cell lines .174/T2 and RMA-S. 35S-Met-labeled class I molecules which have been stabilized by peptides in vitro are immunoprecipitated with conformation dependent monoclonal antibod...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
1993
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