A method to quantify binding of unlabeled peptides to class I MHC molecules and detect their allele specificity.

A general method has been developed for measuring the stabilization of class I MHC molecules in extracts of the mutant cell lines .174/T2 and RMA-S. 35S-Met-labeled class I molecules which have been stabilized by peptides in vitro are immunoprecipitated with conformation dependent monoclonal antibod...

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Bibliographic Details
Main Authors: Elvin, J, Potter, C, Elliott, T, Cerundolo, V, Townsend, A
Format: Journal article
Language:English
Published: 1993

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