T7 RNA polymerase functions in vitro without clustering.
Many nucleic acid polymerases function in clusters known as factories. We investigate whether the RNA polymerase (RNAP) of phage T7 also clusters when active. Using 'pulldowns' and fluorescence correlation spectroscopy we find that elongation complexes do not interact in vitro with a K(d)&...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
Public Library of Science
2012
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