Detecting cAMP-induced Epac activation by fluorescence resonance energy transfer: Epac as a novel cAMP indicator.

Detecting cAMP-induced Epac activation by fluorescence resonance energy transfer: Epac as a novel cAMP indicator.

Epac1 is a guanine nucleotide exchange factor for Rap1 that is activated by direct binding of cAMP. In vitro studies suggest that cAMP relieves the interaction between the regulatory and catalytic domains of Epac. Here, we monitor Epac1 activation in vivo by using a CFP-Epac-YFP fusion construct. Wh...

Bibliografske podrobnosti
Main Authors:	Ponsioen, B, Zhao, J, Riedl, J, Zwartkruis, F, van der Krogt, G, Zaccolo, M, Moolenaar, W, Bos, J, Jalink, K
Format:	Journal article
Jezik:	English
Izdano:	2004

Podobne knjige/članki

EPAC1 activation by cAMP stabilizes CFTR at the membrane by promoting its interaction with NHERF1
od: Zaccolo, M, et al.
Izdano: (2016)

EPAC1 activation by cAMP stabilizes CFTR at the membrane by promoting its interaction with NHERF1
od: Lobo, MJ, et al.
Izdano: (2016)

Epac is a Rap1 guanine-nucleotide-exchange factor directly activated by cyclic AMP.
od: de Rooij, J, et al.
Izdano: (1998)

Microdomains of cAMP in heart cells
od: Zaccolo, M
Izdano: (2002)

Components of the mitochondrial cAMP signalosome
od: Monterisi, S, et al.
Izdano: (2017)

Measuring dynamic changes in cAMP using fluorescence resonance energy transfer.
od: Evellin, S, et al.
Izdano: (2004)

cAMP signaling in subcellular compartments.
od: Lefkimmiatis, K, et al.
Izdano: (2014)

Imaging cAMP nanodomains in the heart
od: Chao, Y, et al.
Izdano: (2019)

Phosphodiesterases and compartmentalized cAMP signalling in the heart.
od: Zaccolo, M
Izdano: (2006)

Activation of PKA in cell requires higher concentration of cAMP than in vitro: implications for compartmentalization of cAMP signalling
od: Koschinski, A, et al.
Izdano: (2017)

Compartmentalisation of cAMP and Ca(2+) signals.
od: Zaccolo, M, et al.
Izdano: (2002)

Subcellular organisation of the cAMP signalling pathway
od: Zaccolo, M, et al.
Izdano: (2020)

Spatial control of cAMP signalling in health and disease.
od: Zaccolo, M
Izdano: (2011)

Using cAMP sensors to study cardiac nanodomains
od: Schleicher, K, et al.
Izdano: (2018)

Elevation of cAMP promotes an Epac2 dependent, L-type Ca2+-channel coupled, Ca2+-induced Ca2+ release in mouse alpha cells
od: Zhang, Q, et al.
Izdano: (2014)

Phosphodiesterases and subcellular compartmentalized cAMP signaling in the cardiovascular system.
od: Stangherlin, A, et al.
Izdano: (2012)

Imaging the cAMP-dependent signal transduction pathway
od: Zaccolo, M, et al.
Izdano: (2005)

Imaging the cAMP-dependent signal transduction pathway.
od: Zaccolo, M, et al.
Izdano: (2005)

Cardiomyocyte membrane structure and cAMP compartmentation produce anatomical variation in β2AR-cAMP responsiveness in murine hearts
od: Wright, PT, et al.
Izdano: (2018)

cAMP signalling meets mitochondrial compartments.
od: Lefkimmiatis, K
Izdano: (2014)

Molecular basis of the allosteric mechanism of cAMP in the regulatory PKA subunit.
od: Pantano, S, et al.
Izdano: (2005)

Spatiotemporal coupling of cAMP transporter to CFTR chloride channel function in the gut epithelia.
od: Li, C, et al.
Izdano: (2007)

cAMP: From long-range second messenger to nanodomain signalling
od: Musheshe, N, et al.
Izdano: (2017)

Cell entry and cAMP imaging of anthrax edema toxin.
od: Dal Molin, F, et al.
Izdano: (2006)

Intracellular tortuosity underlies slow cAMP diffusion in adult ventricular myocytes
od: Swietach, P, et al.
Izdano: (2016)

Compartmentalized cAMP/PKA signalling regulates cardiac excitation-contraction coupling.
od: Lissandron, V, et al.
Izdano: (2006)

Biochemical characterization and cellular imaging of a novel, membrane permeable fluorescent cAMP analog
od: Moll, D, et al.
Izdano: (2008)

Biochemical characterization and cellular imaging of a novel, membrane permeable fluorescent cAMP analog
od: Moll, D, et al.
Izdano: (2008)

Whole-cell cAMP and PKA activity are epiphenomena, nanodomain signaling matters
od: Bers, DM, et al.
Izdano: (2019)

cAMP and cGMP signaling cross-talk: role of phosphodiesterases and implications for cardiac pathophysiology.
od: Zaccolo, M, et al.
Izdano: (2007)

Discrete microdomains with high concentration of cAMP in stimulated rat neonatal cardiac myocytes.
od: Zaccolo, M, et al.
Izdano: (2002)

PKA microdomain organisation and cAMP handling in healthy and dystrophic muscle in vivo.
od: Röder, I, et al.
Izdano: (2009)

Improvement of a FRET-based indicator for cAMP by linker design and stabilization of donor-acceptor interaction.
od: Lissandron, V, et al.
Izdano: (2005)

Selectivity in enrichment of cAMP-dependent protein kinase regulatory subunits type I and type II and their interactors using modified cAMP affinity resins.
od: Aye, T, et al.
Izdano: (2009)

A toolkit for real-time detection of cAMP: insights into compartmentalized signaling.
od: Berrera, M, et al.
Izdano: (2008)

Regulation of the inflammatory response of vascular endothelial cells by EPAC1.
od: Parnell, E, et al.
Izdano: (2012)

Local modulation of cystic fibrosis conductance regulator: cytoskeleton and compartmentalized cAMP signalling.
od: Monterisi, S, et al.
Izdano: (2013)

cAMP signal transduction in the heart: understanding spatial control for the development of novel therapeutic strategies.
od: Zaccolo, M
Izdano: (2009)

Extracellular calcium and cAMP: second messengers as "third messengers"?
od: Hofer, A, et al.
Izdano: (2007)

Impact of cAMP on the T-cell response to type II collagen.
od: Ozegbe, P, et al.
Izdano: (2004)