Viral vectored transmission blocking vaccines against Plasmodium falciparum
Background: Transmission blocking vaccines (TBVs) target sexual develop¬ment of the parasite within the mosquito and aim to prevent transmission of malaria from one individual to another. Antibodies raised against Pfs48/45, Pfs230 Region C, PfHAP2, and Anopheles gambiae Alanyl Aminopeptidase N1 (Ag...
| Main Authors: | , , , , , |
|---|---|
| Format: | Journal article |
| Published: |
BioMed Central
2010
|
| _version_ | 1797054362271350784 |
|---|---|
| author | Kapulu, M Biswas, S Blagborough, A Gilbert, S Sinden, R Hill, A |
| author_facet | Kapulu, M Biswas, S Blagborough, A Gilbert, S Sinden, R Hill, A |
| author_sort | Kapulu, M |
| collection | OXFORD |
| description | Background: Transmission blocking vaccines (TBVs) target sexual develop¬ment of the parasite within the mosquito and aim to prevent transmission of malaria from one individual to another. Antibodies raised against Pfs48/45, Pfs230 Region C, PfHAP2, and Anopheles gambiae Alanyl Aminopeptidase N1 (AgAPN1) proteins reduce transmission i.e. have transmission blocking activity [1–5]. Recombinant simian Adenovirus (AdC63 serotype) and Modified Vaccinia Ankara (MVA) viral vectors have been shown to induce high antibody titres to asexual parasite antigens in animal studies [6]. Materials and methods: Protein sequences for each of the antigens were codon optimised for expression in humans and cloned into shuttle vectors, which were then recombined with the parental virus and purified to obtain virus expressing the antigen of interest. Mice were vaccinated with AdC63 (i.m.), sera was taken after 2 weeks, and will be followed by an MVA boost (i.d.) eight weeks after the prime. Antibodies were assayed by a standardised ELISA, and transmission blocking activity assessed using a standardised membrane feeding assay (SMFA). Conclusion: Induction of high antibody tires using this vaccine platform could be used together with other control measures to achieve elimination and/or eradication of the disease at a local or national level. |
| first_indexed | 2024-03-06T18:56:13Z |
| format | Journal article |
| id | oxford-uuid:11ef380a-6c00-47c7-9503-397f6e42243b |
| institution | University of Oxford |
| last_indexed | 2024-03-06T18:56:13Z |
| publishDate | 2010 |
| publisher | BioMed Central |
| record_format | dspace |
| spelling | oxford-uuid:11ef380a-6c00-47c7-9503-397f6e42243b2022-03-26T10:04:58ZViral vectored transmission blocking vaccines against Plasmodium falciparumJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:11ef380a-6c00-47c7-9503-397f6e42243bSymplectic Elements at OxfordBioMed Central2010Kapulu, MBiswas, SBlagborough, AGilbert, SSinden, RHill, ABackground: Transmission blocking vaccines (TBVs) target sexual develop¬ment of the parasite within the mosquito and aim to prevent transmission of malaria from one individual to another. Antibodies raised against Pfs48/45, Pfs230 Region C, PfHAP2, and Anopheles gambiae Alanyl Aminopeptidase N1 (AgAPN1) proteins reduce transmission i.e. have transmission blocking activity [1–5]. Recombinant simian Adenovirus (AdC63 serotype) and Modified Vaccinia Ankara (MVA) viral vectors have been shown to induce high antibody titres to asexual parasite antigens in animal studies [6]. Materials and methods: Protein sequences for each of the antigens were codon optimised for expression in humans and cloned into shuttle vectors, which were then recombined with the parental virus and purified to obtain virus expressing the antigen of interest. Mice were vaccinated with AdC63 (i.m.), sera was taken after 2 weeks, and will be followed by an MVA boost (i.d.) eight weeks after the prime. Antibodies were assayed by a standardised ELISA, and transmission blocking activity assessed using a standardised membrane feeding assay (SMFA). Conclusion: Induction of high antibody tires using this vaccine platform could be used together with other control measures to achieve elimination and/or eradication of the disease at a local or national level. |
| spellingShingle | Kapulu, M Biswas, S Blagborough, A Gilbert, S Sinden, R Hill, A Viral vectored transmission blocking vaccines against Plasmodium falciparum |
| title | Viral vectored transmission blocking vaccines against Plasmodium falciparum |
| title_full | Viral vectored transmission blocking vaccines against Plasmodium falciparum |
| title_fullStr | Viral vectored transmission blocking vaccines against Plasmodium falciparum |
| title_full_unstemmed | Viral vectored transmission blocking vaccines against Plasmodium falciparum |
| title_short | Viral vectored transmission blocking vaccines against Plasmodium falciparum |
| title_sort | viral vectored transmission blocking vaccines against plasmodium falciparum |
| work_keys_str_mv | AT kapulum viralvectoredtransmissionblockingvaccinesagainstplasmodiumfalciparum AT biswass viralvectoredtransmissionblockingvaccinesagainstplasmodiumfalciparum AT blagborougha viralvectoredtransmissionblockingvaccinesagainstplasmodiumfalciparum AT gilberts viralvectoredtransmissionblockingvaccinesagainstplasmodiumfalciparum AT sindenr viralvectoredtransmissionblockingvaccinesagainstplasmodiumfalciparum AT hilla viralvectoredtransmissionblockingvaccinesagainstplasmodiumfalciparum |