The motility and chemosensory systems of Rhizobium leguminosarum, their role in symbiosis, and link to PTSNtr regulation
<p>Motility and chemotaxis are crucial processes for soil bacteria and plant–microbe interactions. This applies to the symbiotic bacterium <em>Rhizobium leguminosarum</em>, where motility is driven by flagella rotation controlled by two chemotaxis systems, Che1 a...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
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Wiley
2024
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| _version_ | 1797112668940664832 |
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| author | Aroney, STN Pini, F Kessler, C Poole, PS Sánchez-Cañizares, C |
| author_facet | Aroney, STN Pini, F Kessler, C Poole, PS Sánchez-Cañizares, C |
| author_sort | Aroney, STN |
| collection | OXFORD |
| description | <p>Motility and chemotaxis are crucial processes for soil bacteria and plant–microbe interactions. This applies to the symbiotic bacterium <em>Rhizobium leguminosarum</em>, where motility is driven by flagella rotation controlled by two chemotaxis systems, Che1 and Che2. The Che1 cluster is particularly important in free-living motility prior to the establishment of the symbiosis, with a <em>che1</em> mutant delayed in nodulation and reduced in nodulation competitiveness. The Che2 system alters bacteroid development and nodule maturation. In this work, we also identified 27 putative chemoreceptors encoded in the <em>R. leguminosarum bv. viciae</em> 3841 genome and characterized its motility in different growth conditions. We describe a metabolism-based taxis system in rhizobia that acts at high concentrations of dicarboxylates to halt motility independent of chemotaxis. Finally, we show how PTS<sup>Ntr</sup> influences cell motility, with PTS<sup>Ntr</sup> mutants exhibiting reduced swimming in different media. Motility is restored by the active forms of the PTS<sup>Ntr</sup> output regulatory proteins, unphosphorylated ManX and phosphorylated PtsN. Overall, this work shows how rhizobia typify soil bacteria by having a high number of chemoreceptors and highlights the importance of the motility and chemotaxis mechanisms in a free-living cell in the rhizosphere, and at different stages of the symbiosis.</p> |
| first_indexed | 2024-03-07T08:27:07Z |
| format | Journal article |
| id | oxford-uuid:12371829-ac6e-4777-bf36-5958d7b3b2cd |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T08:27:07Z |
| publishDate | 2024 |
| publisher | Wiley |
| record_format | dspace |
| spelling | oxford-uuid:12371829-ac6e-4777-bf36-5958d7b3b2cd2024-02-27T12:00:11ZThe motility and chemosensory systems of Rhizobium leguminosarum, their role in symbiosis, and link to PTSNtr regulationJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:12371829-ac6e-4777-bf36-5958d7b3b2cdEnglishSymplectic ElementsWiley2024Aroney, STNPini, FKessler, CPoole, PSSánchez-Cañizares, C<p>Motility and chemotaxis are crucial processes for soil bacteria and plant–microbe interactions. This applies to the symbiotic bacterium <em>Rhizobium leguminosarum</em>, where motility is driven by flagella rotation controlled by two chemotaxis systems, Che1 and Che2. The Che1 cluster is particularly important in free-living motility prior to the establishment of the symbiosis, with a <em>che1</em> mutant delayed in nodulation and reduced in nodulation competitiveness. The Che2 system alters bacteroid development and nodule maturation. In this work, we also identified 27 putative chemoreceptors encoded in the <em>R. leguminosarum bv. viciae</em> 3841 genome and characterized its motility in different growth conditions. We describe a metabolism-based taxis system in rhizobia that acts at high concentrations of dicarboxylates to halt motility independent of chemotaxis. Finally, we show how PTS<sup>Ntr</sup> influences cell motility, with PTS<sup>Ntr</sup> mutants exhibiting reduced swimming in different media. Motility is restored by the active forms of the PTS<sup>Ntr</sup> output regulatory proteins, unphosphorylated ManX and phosphorylated PtsN. Overall, this work shows how rhizobia typify soil bacteria by having a high number of chemoreceptors and highlights the importance of the motility and chemotaxis mechanisms in a free-living cell in the rhizosphere, and at different stages of the symbiosis.</p> |
| spellingShingle | Aroney, STN Pini, F Kessler, C Poole, PS Sánchez-Cañizares, C The motility and chemosensory systems of Rhizobium leguminosarum, their role in symbiosis, and link to PTSNtr regulation |
| title | The motility and chemosensory systems of Rhizobium leguminosarum, their role in symbiosis, and link to PTSNtr regulation |
| title_full | The motility and chemosensory systems of Rhizobium leguminosarum, their role in symbiosis, and link to PTSNtr regulation |
| title_fullStr | The motility and chemosensory systems of Rhizobium leguminosarum, their role in symbiosis, and link to PTSNtr regulation |
| title_full_unstemmed | The motility and chemosensory systems of Rhizobium leguminosarum, their role in symbiosis, and link to PTSNtr regulation |
| title_short | The motility and chemosensory systems of Rhizobium leguminosarum, their role in symbiosis, and link to PTSNtr regulation |
| title_sort | motility and chemosensory systems of rhizobium leguminosarum their role in symbiosis and link to ptsntr regulation |
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