Identification and drug binding capabilities of tubulin in the nematode Ascaridia galli.
Cell extracts of Ascaridia galli bind colchicine in a manner suggesting the presence of a tubulin-like protein. Column chromatography of these extracts on DEAE-Sephadex yielded only one peak with colchicine-binding activity. Single peaks of radioactivity in this same position were obtained on chroma...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
1982
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| _version_ | 1797054517985935360 |
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| author | Ireland, C Clayton, L Gutteridge, W Pogson, C Gull, K |
| author_facet | Ireland, C Clayton, L Gutteridge, W Pogson, C Gull, K |
| author_sort | Ireland, C |
| collection | OXFORD |
| description | Cell extracts of Ascaridia galli bind colchicine in a manner suggesting the presence of a tubulin-like protein. Column chromatography of these extracts on DEAE-Sephadex yielded only one peak with colchicine-binding activity. Single peaks of radioactivity in this same position were obtained on chromatography of extracts prelabelled with either [3H]colchicine or [3H]parbendazole. Sodium dodecyl sulphate polyacrylamide gel electrophoresis and two dimensional gel electrophoresis of the fractions making up the peaks indicated the presence of two proteins which co-migrate with mammalian brain alpha- and beta-tubulin markers. More detailed investigation showed that the A. galli tubulin has a slightly different alpha-subunit when compared with mammalian tubulin. |
| first_indexed | 2024-03-06T18:58:18Z |
| format | Journal article |
| id | oxford-uuid:12a3e66c-009f-49f1-82c8-01b088e193b4 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T18:58:18Z |
| publishDate | 1982 |
| record_format | dspace |
| spelling | oxford-uuid:12a3e66c-009f-49f1-82c8-01b088e193b42022-03-26T10:08:59ZIdentification and drug binding capabilities of tubulin in the nematode Ascaridia galli.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:12a3e66c-009f-49f1-82c8-01b088e193b4EnglishSymplectic Elements at Oxford1982Ireland, CClayton, LGutteridge, WPogson, CGull, KCell extracts of Ascaridia galli bind colchicine in a manner suggesting the presence of a tubulin-like protein. Column chromatography of these extracts on DEAE-Sephadex yielded only one peak with colchicine-binding activity. Single peaks of radioactivity in this same position were obtained on chromatography of extracts prelabelled with either [3H]colchicine or [3H]parbendazole. Sodium dodecyl sulphate polyacrylamide gel electrophoresis and two dimensional gel electrophoresis of the fractions making up the peaks indicated the presence of two proteins which co-migrate with mammalian brain alpha- and beta-tubulin markers. More detailed investigation showed that the A. galli tubulin has a slightly different alpha-subunit when compared with mammalian tubulin. |
| spellingShingle | Ireland, C Clayton, L Gutteridge, W Pogson, C Gull, K Identification and drug binding capabilities of tubulin in the nematode Ascaridia galli. |
| title | Identification and drug binding capabilities of tubulin in the nematode Ascaridia galli. |
| title_full | Identification and drug binding capabilities of tubulin in the nematode Ascaridia galli. |
| title_fullStr | Identification and drug binding capabilities of tubulin in the nematode Ascaridia galli. |
| title_full_unstemmed | Identification and drug binding capabilities of tubulin in the nematode Ascaridia galli. |
| title_short | Identification and drug binding capabilities of tubulin in the nematode Ascaridia galli. |
| title_sort | identification and drug binding capabilities of tubulin in the nematode ascaridia galli |
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