Label-free single molecule imaging with numerical aperture-shaped interferometric scattering microscopy
Our ability to optically interrogate nanoscopic objects is controlled by the difference between their extinction cross sections and the diffraction-limited area to which light can be confined in the far-field. Here, we show that a partially transmissive spatial mask placed near the back focal plane...
| Autori principali: | , , , |
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| Natura: | Journal article |
| Pubblicazione: |
American Chemical Society
2017
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| _version_ | 1826260034723512320 |
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| author | Cole, D Young, G Weigel, A Kukura, P |
| author_facet | Cole, D Young, G Weigel, A Kukura, P |
| author_sort | Cole, D |
| collection | OXFORD |
| description | Our ability to optically interrogate nanoscopic objects is controlled by the difference between their extinction cross sections and the diffraction-limited area to which light can be confined in the far-field. Here, we show that a partially transmissive spatial mask placed near the back focal plane of a high numerical aperture microscope objective enhances the extinction contrast of a scatterer near an interface by approximately T^-1/2, where T is the transmissivity of the mask. Numerical aperture-based differentiation of background from scattered light represents a general approach to increasing extinction contrast and enables routine label-free imaging down to the single molecule level. |
| first_indexed | 2024-03-06T18:59:13Z |
| format | Journal article |
| id | oxford-uuid:12f1b6dd-3de4-47a7-af7b-569a12c674de |
| institution | University of Oxford |
| last_indexed | 2024-03-06T18:59:13Z |
| publishDate | 2017 |
| publisher | American Chemical Society |
| record_format | dspace |
| spelling | oxford-uuid:12f1b6dd-3de4-47a7-af7b-569a12c674de2022-03-26T10:10:56ZLabel-free single molecule imaging with numerical aperture-shaped interferometric scattering microscopyJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:12f1b6dd-3de4-47a7-af7b-569a12c674deSymplectic Elements at OxfordAmerican Chemical Society2017Cole, DYoung, GWeigel, AKukura, POur ability to optically interrogate nanoscopic objects is controlled by the difference between their extinction cross sections and the diffraction-limited area to which light can be confined in the far-field. Here, we show that a partially transmissive spatial mask placed near the back focal plane of a high numerical aperture microscope objective enhances the extinction contrast of a scatterer near an interface by approximately T^-1/2, where T is the transmissivity of the mask. Numerical aperture-based differentiation of background from scattered light represents a general approach to increasing extinction contrast and enables routine label-free imaging down to the single molecule level. |
| spellingShingle | Cole, D Young, G Weigel, A Kukura, P Label-free single molecule imaging with numerical aperture-shaped interferometric scattering microscopy |
| title | Label-free single molecule imaging with numerical aperture-shaped interferometric scattering microscopy |
| title_full | Label-free single molecule imaging with numerical aperture-shaped interferometric scattering microscopy |
| title_fullStr | Label-free single molecule imaging with numerical aperture-shaped interferometric scattering microscopy |
| title_full_unstemmed | Label-free single molecule imaging with numerical aperture-shaped interferometric scattering microscopy |
| title_short | Label-free single molecule imaging with numerical aperture-shaped interferometric scattering microscopy |
| title_sort | label free single molecule imaging with numerical aperture shaped interferometric scattering microscopy |
| work_keys_str_mv | AT coled labelfreesinglemoleculeimagingwithnumericalapertureshapedinterferometricscatteringmicroscopy AT youngg labelfreesinglemoleculeimagingwithnumericalapertureshapedinterferometricscatteringmicroscopy AT weigela labelfreesinglemoleculeimagingwithnumericalapertureshapedinterferometricscatteringmicroscopy AT kukurap labelfreesinglemoleculeimagingwithnumericalapertureshapedinterferometricscatteringmicroscopy |