Secreted proteins defy the expression level–evolutionary rate anticorrelation
The rates of evolution of the proteins of any organism vary across orders of magnitude. A primary factor influencing rates of protein evolution is expression. A strong negative correlation between expression levels and evolutionary rates (the so-called E–R anticorrelation) has been observed in virtu...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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Oxford University Press
2016
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| _version_ | 1826260035939860480 |
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| author | Feyertag, F Berninsone, P Alvarez-Ponce, D |
| author_facet | Feyertag, F Berninsone, P Alvarez-Ponce, D |
| author_sort | Feyertag, F |
| collection | OXFORD |
| description | The rates of evolution of the proteins of any organism vary across orders of magnitude. A primary factor influencing rates of protein evolution is expression. A strong negative correlation between expression levels and evolutionary rates (the so-called E–R anticorrelation) has been observed in virtually all studied organisms. This effect is currently attributed to the abundance-dependent fitness costs of misfolding and unspecific protein–protein interactions, among other factors. Secreted proteins are folded in the endoplasmic reticulum, a compartment where chaperones, folding catalysts, and stringent quality control mechanisms promote their correct folding and may reduce the fitness costs of misfolding. In addition, confinement of secreted proteins to the extracellular space may reduce misinteractions and their deleterious effects. We hypothesize that each of these factors (the secretory pathway quality control and extracellular location) may reduce the strength of the E–R anticorrelation. Indeed, here we show that among human proteins that are secreted to the extracellular space, rates of evolution do not correlate with protein abundances. This trend is robust to controlling for several potentially confounding factors and is also observed when analyzing protein abundance data for 6 human tissues. In addition, analysis of mRNA abundance data for 32 human tissues shows that the E–R correlation is always less negative, and sometimes nonsignificant, in secreted proteins. Similar observations were made in Caenorhabditis elegans and in Escherichia coli, and to a lesser extent in Drosophila melanogaster, Saccharomyces cerevisiae and Arabidopsis thaliana. Our observations contribute to understand the causes of the E–R anticorrelation. |
| first_indexed | 2024-03-06T18:59:14Z |
| format | Journal article |
| id | oxford-uuid:12f27c2c-52df-45eb-9fa1-fd30e1392433 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T18:59:14Z |
| publishDate | 2016 |
| publisher | Oxford University Press |
| record_format | dspace |
| spelling | oxford-uuid:12f27c2c-52df-45eb-9fa1-fd30e13924332022-03-26T10:10:58ZSecreted proteins defy the expression level–evolutionary rate anticorrelationJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:12f27c2c-52df-45eb-9fa1-fd30e1392433EnglishSymplectic Elements at OxfordOxford University Press2016Feyertag, FBerninsone, PAlvarez-Ponce, DThe rates of evolution of the proteins of any organism vary across orders of magnitude. A primary factor influencing rates of protein evolution is expression. A strong negative correlation between expression levels and evolutionary rates (the so-called E–R anticorrelation) has been observed in virtually all studied organisms. This effect is currently attributed to the abundance-dependent fitness costs of misfolding and unspecific protein–protein interactions, among other factors. Secreted proteins are folded in the endoplasmic reticulum, a compartment where chaperones, folding catalysts, and stringent quality control mechanisms promote their correct folding and may reduce the fitness costs of misfolding. In addition, confinement of secreted proteins to the extracellular space may reduce misinteractions and their deleterious effects. We hypothesize that each of these factors (the secretory pathway quality control and extracellular location) may reduce the strength of the E–R anticorrelation. Indeed, here we show that among human proteins that are secreted to the extracellular space, rates of evolution do not correlate with protein abundances. This trend is robust to controlling for several potentially confounding factors and is also observed when analyzing protein abundance data for 6 human tissues. In addition, analysis of mRNA abundance data for 32 human tissues shows that the E–R correlation is always less negative, and sometimes nonsignificant, in secreted proteins. Similar observations were made in Caenorhabditis elegans and in Escherichia coli, and to a lesser extent in Drosophila melanogaster, Saccharomyces cerevisiae and Arabidopsis thaliana. Our observations contribute to understand the causes of the E–R anticorrelation. |
| spellingShingle | Feyertag, F Berninsone, P Alvarez-Ponce, D Secreted proteins defy the expression level–evolutionary rate anticorrelation |
| title | Secreted proteins defy the expression level–evolutionary rate anticorrelation |
| title_full | Secreted proteins defy the expression level–evolutionary rate anticorrelation |
| title_fullStr | Secreted proteins defy the expression level–evolutionary rate anticorrelation |
| title_full_unstemmed | Secreted proteins defy the expression level–evolutionary rate anticorrelation |
| title_short | Secreted proteins defy the expression level–evolutionary rate anticorrelation |
| title_sort | secreted proteins defy the expression level evolutionary rate anticorrelation |
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