Field evaluation of a novel colorimetric method--double-site enzyme-linked lactate dehydrogenase immunodetection assay--to determine drug susceptibilities of Plasmodium falciparum clinical isolates from northwestern Thailand.
A double-site enzyme-linked lactate dehydrogenase enzyme immunodetection assay was tested against field isolates of Plasmodium falciparum for assessing in vitro drug susceptibilities to a wide range of antimalarial drugs. Its sensitivity allowed the use of parasite densities as low as 200 parasites/...
Egile Nagusiak: | , , , , , |
---|---|
Formatua: | Journal article |
Hizkuntza: | English |
Argitaratua: |
2004
|
_version_ | 1826260036757749760 |
---|---|
author | Brockman, A Singlam, S Phiaphun, L Looareesuwan, S White, N Nosten, F |
author_facet | Brockman, A Singlam, S Phiaphun, L Looareesuwan, S White, N Nosten, F |
author_sort | Brockman, A |
collection | OXFORD |
description | A double-site enzyme-linked lactate dehydrogenase enzyme immunodetection assay was tested against field isolates of Plasmodium falciparum for assessing in vitro drug susceptibilities to a wide range of antimalarial drugs. Its sensitivity allowed the use of parasite densities as low as 200 parasites/microl of blood. Being a nonisotopic, colorimetric assay, it lies within the capabilities of a modest laboratory at the district level. |
first_indexed | 2024-03-06T18:59:16Z |
format | Journal article |
id | oxford-uuid:12f3b760-a967-4acd-b129-09a3043e248c |
institution | University of Oxford |
language | English |
last_indexed | 2024-03-06T18:59:16Z |
publishDate | 2004 |
record_format | dspace |
spelling | oxford-uuid:12f3b760-a967-4acd-b129-09a3043e248c2022-03-26T10:11:01ZField evaluation of a novel colorimetric method--double-site enzyme-linked lactate dehydrogenase immunodetection assay--to determine drug susceptibilities of Plasmodium falciparum clinical isolates from northwestern Thailand.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:12f3b760-a967-4acd-b129-09a3043e248cEnglishSymplectic Elements at Oxford2004Brockman, ASinglam, SPhiaphun, LLooareesuwan, SWhite, NNosten, FA double-site enzyme-linked lactate dehydrogenase enzyme immunodetection assay was tested against field isolates of Plasmodium falciparum for assessing in vitro drug susceptibilities to a wide range of antimalarial drugs. Its sensitivity allowed the use of parasite densities as low as 200 parasites/microl of blood. Being a nonisotopic, colorimetric assay, it lies within the capabilities of a modest laboratory at the district level. |
spellingShingle | Brockman, A Singlam, S Phiaphun, L Looareesuwan, S White, N Nosten, F Field evaluation of a novel colorimetric method--double-site enzyme-linked lactate dehydrogenase immunodetection assay--to determine drug susceptibilities of Plasmodium falciparum clinical isolates from northwestern Thailand. |
title | Field evaluation of a novel colorimetric method--double-site enzyme-linked lactate dehydrogenase immunodetection assay--to determine drug susceptibilities of Plasmodium falciparum clinical isolates from northwestern Thailand. |
title_full | Field evaluation of a novel colorimetric method--double-site enzyme-linked lactate dehydrogenase immunodetection assay--to determine drug susceptibilities of Plasmodium falciparum clinical isolates from northwestern Thailand. |
title_fullStr | Field evaluation of a novel colorimetric method--double-site enzyme-linked lactate dehydrogenase immunodetection assay--to determine drug susceptibilities of Plasmodium falciparum clinical isolates from northwestern Thailand. |
title_full_unstemmed | Field evaluation of a novel colorimetric method--double-site enzyme-linked lactate dehydrogenase immunodetection assay--to determine drug susceptibilities of Plasmodium falciparum clinical isolates from northwestern Thailand. |
title_short | Field evaluation of a novel colorimetric method--double-site enzyme-linked lactate dehydrogenase immunodetection assay--to determine drug susceptibilities of Plasmodium falciparum clinical isolates from northwestern Thailand. |
title_sort | field evaluation of a novel colorimetric method double site enzyme linked lactate dehydrogenase immunodetection assay to determine drug susceptibilities of plasmodium falciparum clinical isolates from northwestern thailand |
work_keys_str_mv | AT brockmana fieldevaluationofanovelcolorimetricmethoddoublesiteenzymelinkedlactatedehydrogenaseimmunodetectionassaytodeterminedrugsusceptibilitiesofplasmodiumfalciparumclinicalisolatesfromnorthwesternthailand AT singlams fieldevaluationofanovelcolorimetricmethoddoublesiteenzymelinkedlactatedehydrogenaseimmunodetectionassaytodeterminedrugsusceptibilitiesofplasmodiumfalciparumclinicalisolatesfromnorthwesternthailand AT phiaphunl fieldevaluationofanovelcolorimetricmethoddoublesiteenzymelinkedlactatedehydrogenaseimmunodetectionassaytodeterminedrugsusceptibilitiesofplasmodiumfalciparumclinicalisolatesfromnorthwesternthailand AT looareesuwans fieldevaluationofanovelcolorimetricmethoddoublesiteenzymelinkedlactatedehydrogenaseimmunodetectionassaytodeterminedrugsusceptibilitiesofplasmodiumfalciparumclinicalisolatesfromnorthwesternthailand AT whiten fieldevaluationofanovelcolorimetricmethoddoublesiteenzymelinkedlactatedehydrogenaseimmunodetectionassaytodeterminedrugsusceptibilitiesofplasmodiumfalciparumclinicalisolatesfromnorthwesternthailand AT nostenf fieldevaluationofanovelcolorimetricmethoddoublesiteenzymelinkedlactatedehydrogenaseimmunodetectionassaytodeterminedrugsusceptibilitiesofplasmodiumfalciparumclinicalisolatesfromnorthwesternthailand |