Free-floating epithelial micro-tissue arrays: A low cost and versatile technique
Three dimensional (3D) tissue models are invaluable tools that can closely reflect the in vivo physiological environment. However, they are usually difficult to develop, have a low throughput and are often costly; limiting their utility to most laboratories. The recent availability of inexpensive ad...
| Príomhchruthaitheoirí: | , , |
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| Formáid: | Journal article |
| Foilsithe / Cruthaithe: |
IOP Publishing
2016
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| _version_ | 1826260345865371648 |
|---|---|
| author | Flood, P Alvarez, L Reynaud, E |
| author_facet | Flood, P Alvarez, L Reynaud, E |
| author_sort | Flood, P |
| collection | OXFORD |
| description | Three dimensional (3D) tissue models are invaluable tools that can closely reflect the in vivo physiological environment. However, they are usually difficult to develop, have a low throughput and are often costly; limiting their utility to most laboratories. The recent availability of inexpensive additive manufacturing printers and open source 3D design software offers us the possibility to easily create affordable 3D cell culture platforms. To demonstrate this, we established a simple, inexpensive and robust method for producing arrays of free-floating epithelial micro-tissues. Using a combination of 3D Computer Aided Design and 3D printing, hydrogel micro-moulding and collagen cell encapsulation we engineered microenvironments that consistently direct the growth of micro-tissue arrays. We described the adaptability of this technique by testing several immortalized epithelial cell lines and by generating branching morphology and micron to millimetre scaled micro-tissues. We established by fluorescence and electron microscopy that micro-tissues are polarized, have cell type specific differentiated phenotypes and regain native in vivo tissue qualities. Finally, using Salmonella typhimurium we show micro-tissues display a more physiologically relevant infection response compared to other epithelial models. In summary, we have developed a robust and adaptable technique for producing arrays of epithelial micro-tissues. This in vitro model has the potential to be a valuable tool for studying epithelial cell and tissue function/architecture in a physiologically relevant context. |
| first_indexed | 2024-03-06T19:04:10Z |
| format | Journal article |
| id | oxford-uuid:1490f84f-ec69-4714-8ff5-bc088014c6bb |
| institution | University of Oxford |
| last_indexed | 2024-03-06T19:04:10Z |
| publishDate | 2016 |
| publisher | IOP Publishing |
| record_format | dspace |
| spelling | oxford-uuid:1490f84f-ec69-4714-8ff5-bc088014c6bb2022-03-26T10:20:29ZFree-floating epithelial micro-tissue arrays: A low cost and versatile techniqueJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:1490f84f-ec69-4714-8ff5-bc088014c6bbSymplectic Elements at OxfordIOP Publishing2016Flood, PAlvarez, LReynaud, EThree dimensional (3D) tissue models are invaluable tools that can closely reflect the in vivo physiological environment. However, they are usually difficult to develop, have a low throughput and are often costly; limiting their utility to most laboratories. The recent availability of inexpensive additive manufacturing printers and open source 3D design software offers us the possibility to easily create affordable 3D cell culture platforms. To demonstrate this, we established a simple, inexpensive and robust method for producing arrays of free-floating epithelial micro-tissues. Using a combination of 3D Computer Aided Design and 3D printing, hydrogel micro-moulding and collagen cell encapsulation we engineered microenvironments that consistently direct the growth of micro-tissue arrays. We described the adaptability of this technique by testing several immortalized epithelial cell lines and by generating branching morphology and micron to millimetre scaled micro-tissues. We established by fluorescence and electron microscopy that micro-tissues are polarized, have cell type specific differentiated phenotypes and regain native in vivo tissue qualities. Finally, using Salmonella typhimurium we show micro-tissues display a more physiologically relevant infection response compared to other epithelial models. In summary, we have developed a robust and adaptable technique for producing arrays of epithelial micro-tissues. This in vitro model has the potential to be a valuable tool for studying epithelial cell and tissue function/architecture in a physiologically relevant context. |
| spellingShingle | Flood, P Alvarez, L Reynaud, E Free-floating epithelial micro-tissue arrays: A low cost and versatile technique |
| title | Free-floating epithelial micro-tissue arrays: A low cost and versatile technique |
| title_full | Free-floating epithelial micro-tissue arrays: A low cost and versatile technique |
| title_fullStr | Free-floating epithelial micro-tissue arrays: A low cost and versatile technique |
| title_full_unstemmed | Free-floating epithelial micro-tissue arrays: A low cost and versatile technique |
| title_short | Free-floating epithelial micro-tissue arrays: A low cost and versatile technique |
| title_sort | free floating epithelial micro tissue arrays a low cost and versatile technique |
| work_keys_str_mv | AT floodp freefloatingepithelialmicrotissuearraysalowcostandversatiletechnique AT alvarezl freefloatingepithelialmicrotissuearraysalowcostandversatiletechnique AT reynaude freefloatingepithelialmicrotissuearraysalowcostandversatiletechnique |