Bacterial competition systems share a domain required for inner membrane transport of the bacteriocin pyocin G from Pseudomonas aeruginosa
Bacteria exploit a variety of attack strategies to gain dominance within ecological niches. Prominent among these are contact-dependent inhibition (CDI), type VI secretion (T6SS), and bacteriocins. The cytotoxic endpoint of these systems is often the delivery of a nuclease to the cytosol. How such n...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
American Society for Microbiology
2022
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| _version_ | 1797107910924304384 |
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| author | Atanaskovic, I Sharp, C Press, P Kaminska, R Kleanthous, K |
| author_facet | Atanaskovic, I Sharp, C Press, P Kaminska, R Kleanthous, K |
| author_sort | Atanaskovic, I |
| collection | OXFORD |
| description | Bacteria exploit a variety of attack strategies to gain dominance within ecological niches. Prominent among these are contact-dependent inhibition (CDI), type VI secretion (T6SS), and bacteriocins. The cytotoxic endpoint of these systems is often the delivery of a nuclease to the cytosol. How such nucleases translocate across the cytoplasmic membrane of Gram-negative bacteria is unknown. Here, we identify a small, conserved, 15-kDa domain, which we refer to as the inner membrane translocation (IMT) domain, that is common to T6SS and bacteriocins and linked to nuclease effector domains. Through fluorescence microscopy assays using intact and spheroplasted cells, we demonstrate that the IMT domain of the Pseudomonas aeruginosa-specific bacteriocin pyocin G (PyoG) is required for import of the toxin nuclease domain to the cytoplasm. We also show that translocation of PyoG into the cytosol is dependent on inner membrane proteins FtsH, a AAA+ATPase/protease, and TonB1, the latter more typically associated with transport of bacteriocins across the outer membrane. Our study reveals that the IMT domain directs the cytotoxic nuclease of PyoG to cross the cytoplasmic membrane and, more broadly, has been adapted for the transport of other toxic nucleases delivered into Gram-negative bacteria by both contact-dependent and contact-independent means. |
| first_indexed | 2024-03-07T07:22:20Z |
| format | Journal article |
| id | oxford-uuid:15bbf275-2849-4a33-955e-0d61d23d7e82 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T07:22:20Z |
| publishDate | 2022 |
| publisher | American Society for Microbiology |
| record_format | dspace |
| spelling | oxford-uuid:15bbf275-2849-4a33-955e-0d61d23d7e822022-10-28T11:50:40ZBacterial competition systems share a domain required for inner membrane transport of the bacteriocin pyocin G from Pseudomonas aeruginosaJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:15bbf275-2849-4a33-955e-0d61d23d7e82EnglishSymplectic ElementsAmerican Society for Microbiology2022Atanaskovic, ISharp, CPress, PKaminska, RKleanthous, KBacteria exploit a variety of attack strategies to gain dominance within ecological niches. Prominent among these are contact-dependent inhibition (CDI), type VI secretion (T6SS), and bacteriocins. The cytotoxic endpoint of these systems is often the delivery of a nuclease to the cytosol. How such nucleases translocate across the cytoplasmic membrane of Gram-negative bacteria is unknown. Here, we identify a small, conserved, 15-kDa domain, which we refer to as the inner membrane translocation (IMT) domain, that is common to T6SS and bacteriocins and linked to nuclease effector domains. Through fluorescence microscopy assays using intact and spheroplasted cells, we demonstrate that the IMT domain of the Pseudomonas aeruginosa-specific bacteriocin pyocin G (PyoG) is required for import of the toxin nuclease domain to the cytoplasm. We also show that translocation of PyoG into the cytosol is dependent on inner membrane proteins FtsH, a AAA+ATPase/protease, and TonB1, the latter more typically associated with transport of bacteriocins across the outer membrane. Our study reveals that the IMT domain directs the cytotoxic nuclease of PyoG to cross the cytoplasmic membrane and, more broadly, has been adapted for the transport of other toxic nucleases delivered into Gram-negative bacteria by both contact-dependent and contact-independent means. |
| spellingShingle | Atanaskovic, I Sharp, C Press, P Kaminska, R Kleanthous, K Bacterial competition systems share a domain required for inner membrane transport of the bacteriocin pyocin G from Pseudomonas aeruginosa |
| title | Bacterial competition systems share a domain required for inner membrane transport of the bacteriocin pyocin G from Pseudomonas aeruginosa |
| title_full | Bacterial competition systems share a domain required for inner membrane transport of the bacteriocin pyocin G from Pseudomonas aeruginosa |
| title_fullStr | Bacterial competition systems share a domain required for inner membrane transport of the bacteriocin pyocin G from Pseudomonas aeruginosa |
| title_full_unstemmed | Bacterial competition systems share a domain required for inner membrane transport of the bacteriocin pyocin G from Pseudomonas aeruginosa |
| title_short | Bacterial competition systems share a domain required for inner membrane transport of the bacteriocin pyocin G from Pseudomonas aeruginosa |
| title_sort | bacterial competition systems share a domain required for inner membrane transport of the bacteriocin pyocin g from pseudomonas aeruginosa |
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