DNA mismatch detection by resonance energy transfer between ruthenium(II) and osmium(II) tris(2,2'-bipyridyl) chromophores.
Octahedral tris-chelate complexes [M(II)(bpy)(3)](2+) (M = Ru or Os, bpy = 2,2'-bipyridyl), covalently attached to the 3'- and 5'-phosphates of two oligonucleotides, are juxtaposed when hybridized contiguously to a fully complementary DNA target. Visible metal-to-ligand charge-transfe...
| Những tác giả chính: | , , , , , , , |
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| Định dạng: | Journal article |
| Ngôn ngữ: | English |
| Được phát hành: |
2005
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| _version_ | 1826260577086865408 |
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| author | Bichenkova, E Yu, X Bhadra, P Heissigerova, H Pope, S Coe, B Faulkner, S Douglas, K |
| author_facet | Bichenkova, E Yu, X Bhadra, P Heissigerova, H Pope, S Coe, B Faulkner, S Douglas, K |
| author_sort | Bichenkova, E |
| collection | OXFORD |
| description | Octahedral tris-chelate complexes [M(II)(bpy)(3)](2+) (M = Ru or Os, bpy = 2,2'-bipyridyl), covalently attached to the 3'- and 5'-phosphates of two oligonucleotides, are juxtaposed when hybridized contiguously to a fully complementary DNA target. Visible metal-to-ligand charge-transfer (MLCT) excitation of the [Ru(II)(bpy)(3)](2+) unit leads to resonance energy transfer to the MLCT state of the [Os(II)(bpy)(3)](2+) moiety, with the energy transfer efficiency depending on the degree of hybridization. The extent of attenuation of the intense red luminescence from the Ru(II) chromophore hence allows highly sensitive structural probing of the assembly and constitutes a novel approach to DNA sensing which is capable of detecting mutations. |
| first_indexed | 2024-03-06T19:07:52Z |
| format | Journal article |
| id | oxford-uuid:15c8503c-6b08-47c1-a961-1e9a97365f71 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T19:07:52Z |
| publishDate | 2005 |
| record_format | dspace |
| spelling | oxford-uuid:15c8503c-6b08-47c1-a961-1e9a97365f712022-03-26T10:27:22ZDNA mismatch detection by resonance energy transfer between ruthenium(II) and osmium(II) tris(2,2'-bipyridyl) chromophores.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:15c8503c-6b08-47c1-a961-1e9a97365f71EnglishSymplectic Elements at Oxford2005Bichenkova, EYu, XBhadra, PHeissigerova, HPope, SCoe, BFaulkner, SDouglas, KOctahedral tris-chelate complexes [M(II)(bpy)(3)](2+) (M = Ru or Os, bpy = 2,2'-bipyridyl), covalently attached to the 3'- and 5'-phosphates of two oligonucleotides, are juxtaposed when hybridized contiguously to a fully complementary DNA target. Visible metal-to-ligand charge-transfer (MLCT) excitation of the [Ru(II)(bpy)(3)](2+) unit leads to resonance energy transfer to the MLCT state of the [Os(II)(bpy)(3)](2+) moiety, with the energy transfer efficiency depending on the degree of hybridization. The extent of attenuation of the intense red luminescence from the Ru(II) chromophore hence allows highly sensitive structural probing of the assembly and constitutes a novel approach to DNA sensing which is capable of detecting mutations. |
| spellingShingle | Bichenkova, E Yu, X Bhadra, P Heissigerova, H Pope, S Coe, B Faulkner, S Douglas, K DNA mismatch detection by resonance energy transfer between ruthenium(II) and osmium(II) tris(2,2'-bipyridyl) chromophores. |
| title | DNA mismatch detection by resonance energy transfer between ruthenium(II) and osmium(II) tris(2,2'-bipyridyl) chromophores. |
| title_full | DNA mismatch detection by resonance energy transfer between ruthenium(II) and osmium(II) tris(2,2'-bipyridyl) chromophores. |
| title_fullStr | DNA mismatch detection by resonance energy transfer between ruthenium(II) and osmium(II) tris(2,2'-bipyridyl) chromophores. |
| title_full_unstemmed | DNA mismatch detection by resonance energy transfer between ruthenium(II) and osmium(II) tris(2,2'-bipyridyl) chromophores. |
| title_short | DNA mismatch detection by resonance energy transfer between ruthenium(II) and osmium(II) tris(2,2'-bipyridyl) chromophores. |
| title_sort | dna mismatch detection by resonance energy transfer between ruthenium ii and osmium ii tris 2 2 bipyridyl chromophores |
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