Identification of novel HIV-1-derived HLA-E-binding peptides
Non-classical class Ib MHC-E molecule is becoming an increasingly interesting component of the immune response. It is involved in both the adaptive and innate immune responses to several chronic infections including HIV-1 and, under very specific circumstances, likely mediated a unique vaccine prote...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
Elsevier
2018
|
| _version_ | 1797055252255473664 |
|---|---|
| author | Hannoun, Z Lin, Z Brackenridge, S Kuse, N Akahoshi, T Borthwick, N McMichael, A Murakoshi, H Takiguchi, M Hanke, T |
| author_facet | Hannoun, Z Lin, Z Brackenridge, S Kuse, N Akahoshi, T Borthwick, N McMichael, A Murakoshi, H Takiguchi, M Hanke, T |
| author_sort | Hannoun, Z |
| collection | OXFORD |
| description | Non-classical class Ib MHC-E molecule is becoming an increasingly interesting component of the immune response. It is involved in both the adaptive and innate immune responses to several chronic infections including HIV-1 and, under very specific circumstances, likely mediated a unique vaccine protection of rhesus macaques against pathogenic SIV challenge. Despite being recently in the spotlight for HIV-1 vaccine development, to date there is only one reported human leukocyte antigen (HLA)-E-binding peptide derived from HIV-1. In an effort to help start understanding the possible functions of HLA-E in HIV-1 infection, we determined novel HLA-E binding peptides derived from HIV-1 Gag, Pol and Vif proteins. These peptides were identified in three independent assays, all quantifying cell-surface stabilization of HLA-E*01:01 or HLA-E*01:03 molecules upon peptide binding, which was detected by HLA-E-specific monoclonal antibody and flow cytometry. Thus, following initial screen of over 400 HIV-1-derived 15-mer peptides, 4 novel 9-mer peptides PM9, RL9, RV9 and TP9 derived from 15-mer binders specifically stabilized surface expression of HLA-E*01:03 on the cell surface in two separate assays and 5 other binding candidates EI9, MD9, NR9, QF9 and YG9 gave a binding signal in only one of the two assays, but not both. Overall, we have expanded the current knowledge of HIV-1-derived target peptides stabilizing HLA-E cell-surface expression from 1 to 5, thus broadening inroads for future studies. This is a small, but significant contribution towards studying the fine mechanisms behind HLA-E actions and their possible use in development of a new kind of vaccines. |
| first_indexed | 2024-03-06T19:08:02Z |
| format | Journal article |
| id | oxford-uuid:15d3f57a-8eb0-41b3-a05c-b0c139f58593 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T19:08:02Z |
| publishDate | 2018 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | oxford-uuid:15d3f57a-8eb0-41b3-a05c-b0c139f585932022-03-26T10:27:44ZIdentification of novel HIV-1-derived HLA-E-binding peptidesJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:15d3f57a-8eb0-41b3-a05c-b0c139f58593EnglishSymplectic Elements at OxfordElsevier2018Hannoun, ZLin, ZBrackenridge, SKuse, NAkahoshi, TBorthwick, NMcMichael, AMurakoshi, HTakiguchi, MHanke, TNon-classical class Ib MHC-E molecule is becoming an increasingly interesting component of the immune response. It is involved in both the adaptive and innate immune responses to several chronic infections including HIV-1 and, under very specific circumstances, likely mediated a unique vaccine protection of rhesus macaques against pathogenic SIV challenge. Despite being recently in the spotlight for HIV-1 vaccine development, to date there is only one reported human leukocyte antigen (HLA)-E-binding peptide derived from HIV-1. In an effort to help start understanding the possible functions of HLA-E in HIV-1 infection, we determined novel HLA-E binding peptides derived from HIV-1 Gag, Pol and Vif proteins. These peptides were identified in three independent assays, all quantifying cell-surface stabilization of HLA-E*01:01 or HLA-E*01:03 molecules upon peptide binding, which was detected by HLA-E-specific monoclonal antibody and flow cytometry. Thus, following initial screen of over 400 HIV-1-derived 15-mer peptides, 4 novel 9-mer peptides PM9, RL9, RV9 and TP9 derived from 15-mer binders specifically stabilized surface expression of HLA-E*01:03 on the cell surface in two separate assays and 5 other binding candidates EI9, MD9, NR9, QF9 and YG9 gave a binding signal in only one of the two assays, but not both. Overall, we have expanded the current knowledge of HIV-1-derived target peptides stabilizing HLA-E cell-surface expression from 1 to 5, thus broadening inroads for future studies. This is a small, but significant contribution towards studying the fine mechanisms behind HLA-E actions and their possible use in development of a new kind of vaccines. |
| spellingShingle | Hannoun, Z Lin, Z Brackenridge, S Kuse, N Akahoshi, T Borthwick, N McMichael, A Murakoshi, H Takiguchi, M Hanke, T Identification of novel HIV-1-derived HLA-E-binding peptides |
| title | Identification of novel HIV-1-derived HLA-E-binding peptides |
| title_full | Identification of novel HIV-1-derived HLA-E-binding peptides |
| title_fullStr | Identification of novel HIV-1-derived HLA-E-binding peptides |
| title_full_unstemmed | Identification of novel HIV-1-derived HLA-E-binding peptides |
| title_short | Identification of novel HIV-1-derived HLA-E-binding peptides |
| title_sort | identification of novel hiv 1 derived hla e binding peptides |
| work_keys_str_mv | AT hannounz identificationofnovelhiv1derivedhlaebindingpeptides AT linz identificationofnovelhiv1derivedhlaebindingpeptides AT brackenridges identificationofnovelhiv1derivedhlaebindingpeptides AT kusen identificationofnovelhiv1derivedhlaebindingpeptides AT akahoshit identificationofnovelhiv1derivedhlaebindingpeptides AT borthwickn identificationofnovelhiv1derivedhlaebindingpeptides AT mcmichaela identificationofnovelhiv1derivedhlaebindingpeptides AT murakoshih identificationofnovelhiv1derivedhlaebindingpeptides AT takiguchim identificationofnovelhiv1derivedhlaebindingpeptides AT hanket identificationofnovelhiv1derivedhlaebindingpeptides |