Activation of glycogen phosphorylase and glycogenolysis in rat skeletal muscle by AICAR — an activator of AMP-activated protein kinase
<p>We determined whether the cell permeable molecule AICAR, whose metabolite activates AMP-activated protein kinase (AMPK) in cells, affected glycogen metabolism in rat seleus muscle preparations in vitro. The basal and insulin-stimulated rates of radiochemical lactate formation, net lactate r...
| Main Authors: | , , |
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| Other Authors: | |
| Format: | Journal article |
| Language: | English |
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Elsevier
1996
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| Subjects: |
| _version_ | 1797055308689833984 |
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| author | Young, M Radda, G Leighton, B |
| author2 | Federation of European Biochemical Societies |
| author_facet | Federation of European Biochemical Societies Young, M Radda, G Leighton, B |
| author_sort | Young, M |
| collection | OXFORD |
| description | <p>We determined whether the cell permeable molecule AICAR, whose metabolite activates AMP-activated protein kinase (AMPK) in cells, affected glycogen metabolism in rat seleus muscle preparations in vitro. The basal and insulin-stimulated rates of radiochemical lactate formation, net lactate release and glycogen synthesis were determined. AICAR stimulated net lactate release (but not radiochemical lactate formation) only at a basal concentration of insulin. An increased rate of glycogenolysis was the likely cause of increased net lactate release as glycogen phosphorylase activity was significantly increased by AICAR. AICAR-stimulated net lactate release and phosphorylase activity were potently inhibited by insulin.</p> |
| first_indexed | 2024-03-06T19:08:50Z |
| format | Journal article |
| id | oxford-uuid:1616cd97-6eea-411c-ac17-ad4899fed69e |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T19:08:50Z |
| publishDate | 1996 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | oxford-uuid:1616cd97-6eea-411c-ac17-ad4899fed69e2022-03-26T10:29:14ZActivation of glycogen phosphorylase and glycogenolysis in rat skeletal muscle by AICAR — an activator of AMP-activated protein kinaseJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:1616cd97-6eea-411c-ac17-ad4899fed69eBiochemistryEnglishOxford University Research Archive - ValetElsevier1996Young, MRadda, GLeighton, BFederation of European Biochemical Societies<p>We determined whether the cell permeable molecule AICAR, whose metabolite activates AMP-activated protein kinase (AMPK) in cells, affected glycogen metabolism in rat seleus muscle preparations in vitro. The basal and insulin-stimulated rates of radiochemical lactate formation, net lactate release and glycogen synthesis were determined. AICAR stimulated net lactate release (but not radiochemical lactate formation) only at a basal concentration of insulin. An increased rate of glycogenolysis was the likely cause of increased net lactate release as glycogen phosphorylase activity was significantly increased by AICAR. AICAR-stimulated net lactate release and phosphorylase activity were potently inhibited by insulin.</p> |
| spellingShingle | Biochemistry Young, M Radda, G Leighton, B Activation of glycogen phosphorylase and glycogenolysis in rat skeletal muscle by AICAR — an activator of AMP-activated protein kinase |
| title | Activation of glycogen phosphorylase and glycogenolysis in rat skeletal muscle by AICAR — an activator of AMP-activated protein kinase |
| title_full | Activation of glycogen phosphorylase and glycogenolysis in rat skeletal muscle by AICAR — an activator of AMP-activated protein kinase |
| title_fullStr | Activation of glycogen phosphorylase and glycogenolysis in rat skeletal muscle by AICAR — an activator of AMP-activated protein kinase |
| title_full_unstemmed | Activation of glycogen phosphorylase and glycogenolysis in rat skeletal muscle by AICAR — an activator of AMP-activated protein kinase |
| title_short | Activation of glycogen phosphorylase and glycogenolysis in rat skeletal muscle by AICAR — an activator of AMP-activated protein kinase |
| title_sort | activation of glycogen phosphorylase and glycogenolysis in rat skeletal muscle by aicar an activator of amp activated protein kinase |
| topic | Biochemistry |
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