Structural studies on oligosaccharides from mammalian glycoproteins
A method for the purification of human serum immunoglobulin Al (IgA1), suitable for subsequent oligosaccharide analysis, was devised. The N- and 0-linked glycans of normal human serum IgA1 were released from the protein and the structures determined by a combination of gas-chromatography mass spect...
| Main Authors: | , |
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| Format: | Thesis |
| Language: | English |
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1989
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| _version_ | 1826315735013523456 |
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| author | Field, M Field, Mark Christian |
| author2 | Dwek, R |
| author_facet | Dwek, R Field, M Field, Mark Christian |
| author_sort | Field, M |
| collection | OXFORD |
| description | A method for the purification of human serum immunoglobulin Al (IgA1), suitable for subsequent oligosaccharide analysis, was devised. The N- and 0-linked glycans of normal human serum IgA1 were released from the protein and the structures determined by a combination of gas-chromatography mass spectrometry, nuclear magnetic resonance spectroscopy and enzymatic degradation. The glycans from serum IgA1 from normal humans and patients with rheumatoid arthritis were compared. Contrary to observations for immunoglobulin G (IgG), no significant alterations in the IgA1 glycans were encountered in the disease state, suggesting that the defect is IgG specific. The antibodies HNK-1 and L2 bind to a number of glycoproteins important in neural cell adhesion and also recognise glycolipids with a sulphonylglucuronic acid residue. The saccharide from these glycolipids influences neural cell adhesion |
| first_indexed | 2024-03-06T19:09:22Z |
| format | Thesis |
| id | oxford-uuid:163fb9d7-43b7-4347-ab81-ce3cb87cb3f9 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-12-09T03:31:27Z |
| publishDate | 1989 |
| record_format | dspace |
| spelling | oxford-uuid:163fb9d7-43b7-4347-ab81-ce3cb87cb3f92024-12-01T14:48:53ZStructural studies on oligosaccharides from mammalian glycoproteinsThesishttp://purl.org/coar/resource_type/c_db06uuid:163fb9d7-43b7-4347-ab81-ce3cb87cb3f9ImmunoglobulinsNervous systemGlycoproteinsOligosaccharidesStructureSerumEnglishPolonsky Theses Digitisation Project1989Field, MField, Mark ChristianDwek, RRademacher, T A method for the purification of human serum immunoglobulin Al (IgA1), suitable for subsequent oligosaccharide analysis, was devised. The N- and 0-linked glycans of normal human serum IgA1 were released from the protein and the structures determined by a combination of gas-chromatography mass spectrometry, nuclear magnetic resonance spectroscopy and enzymatic degradation. The glycans from serum IgA1 from normal humans and patients with rheumatoid arthritis were compared. Contrary to observations for immunoglobulin G (IgG), no significant alterations in the IgA1 glycans were encountered in the disease state, suggesting that the defect is IgG specific. The antibodies HNK-1 and L2 bind to a number of glycoproteins important in neural cell adhesion and also recognise glycolipids with a sulphonylglucuronic acid residue. The saccharide from these glycolipids influences neural cell adhesion |
| spellingShingle | Immunoglobulins Nervous system Glycoproteins Oligosaccharides Structure Serum Field, M Field, Mark Christian Structural studies on oligosaccharides from mammalian glycoproteins |
| title | Structural studies on oligosaccharides from mammalian glycoproteins |
| title_full | Structural studies on oligosaccharides from mammalian glycoproteins |
| title_fullStr | Structural studies on oligosaccharides from mammalian glycoproteins |
| title_full_unstemmed | Structural studies on oligosaccharides from mammalian glycoproteins |
| title_short | Structural studies on oligosaccharides from mammalian glycoproteins |
| title_sort | structural studies on oligosaccharides from mammalian glycoproteins |
| topic | Immunoglobulins Nervous system Glycoproteins Oligosaccharides Structure Serum |
| work_keys_str_mv | AT fieldm structuralstudiesonoligosaccharidesfrommammalianglycoproteins AT fieldmarkchristian structuralstudiesonoligosaccharidesfrommammalianglycoproteins |