Lifetime-identification of single molecules in aqueous solution
Photon bursts of single rhodamine 6G and rhodamine B molecules in aqueous solution were studied by excitation with a frequency-doubled titanium: sapphire laser. Multichannel scalar traces, fluorescence correlation functions and fluorescence decays determined by time- correlated single-photon countin...
| Main Authors: | , , , , , , |
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| Format: | Journal article |
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1997
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| _version_ | 1826260700553543680 |
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| author | Zander, C Sauer, M Drexhage, K Wolfrum, J Brand, L Eggeling, C Seidel, C |
| author_facet | Zander, C Sauer, M Drexhage, K Wolfrum, J Brand, L Eggeling, C Seidel, C |
| author_sort | Zander, C |
| collection | OXFORD |
| description | Photon bursts of single rhodamine 6G and rhodamine B molecules in aqueous solution were studied by excitation with a frequency-doubled titanium: sapphire laser. Multichannel scalar traces, fluorescence correlation functions and fluorescence decays determined by time- correlated single-photon counting have been measured simultaneously. The time-resolved fluorescence signals were analyzed with a maximum likelihood estimator. With the setup described it is possible to distinguish single dye molecules of different kind via their characteristic fluorescence lifetimes of 1.79 +/- 0.33 ns for rhodamine B-zwitterion and 3.79 +/- 0.38 ns for rhodamine 6G. ©2005 Copyright SPIE - The International Society for Optical Engineering. |
| first_indexed | 2024-03-06T19:09:51Z |
| format | Journal article |
| id | oxford-uuid:1666d7cf-9d0b-438e-97e9-511a13b22060 |
| institution | University of Oxford |
| last_indexed | 2024-03-06T19:09:51Z |
| publishDate | 1997 |
| record_format | dspace |
| spelling | oxford-uuid:1666d7cf-9d0b-438e-97e9-511a13b220602022-03-26T10:31:07ZLifetime-identification of single molecules in aqueous solutionJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:1666d7cf-9d0b-438e-97e9-511a13b22060Symplectic Elements at Oxford1997Zander, CSauer, MDrexhage, KWolfrum, JBrand, LEggeling, CSeidel, CPhoton bursts of single rhodamine 6G and rhodamine B molecules in aqueous solution were studied by excitation with a frequency-doubled titanium: sapphire laser. Multichannel scalar traces, fluorescence correlation functions and fluorescence decays determined by time- correlated single-photon counting have been measured simultaneously. The time-resolved fluorescence signals were analyzed with a maximum likelihood estimator. With the setup described it is possible to distinguish single dye molecules of different kind via their characteristic fluorescence lifetimes of 1.79 +/- 0.33 ns for rhodamine B-zwitterion and 3.79 +/- 0.38 ns for rhodamine 6G. ©2005 Copyright SPIE - The International Society for Optical Engineering. |
| spellingShingle | Zander, C Sauer, M Drexhage, K Wolfrum, J Brand, L Eggeling, C Seidel, C Lifetime-identification of single molecules in aqueous solution |
| title | Lifetime-identification of single molecules in aqueous solution |
| title_full | Lifetime-identification of single molecules in aqueous solution |
| title_fullStr | Lifetime-identification of single molecules in aqueous solution |
| title_full_unstemmed | Lifetime-identification of single molecules in aqueous solution |
| title_short | Lifetime-identification of single molecules in aqueous solution |
| title_sort | lifetime identification of single molecules in aqueous solution |
| work_keys_str_mv | AT zanderc lifetimeidentificationofsinglemoleculesinaqueoussolution AT sauerm lifetimeidentificationofsinglemoleculesinaqueoussolution AT drexhagek lifetimeidentificationofsinglemoleculesinaqueoussolution AT wolfrumj lifetimeidentificationofsinglemoleculesinaqueoussolution AT brandl lifetimeidentificationofsinglemoleculesinaqueoussolution AT eggelingc lifetimeidentificationofsinglemoleculesinaqueoussolution AT seidelc lifetimeidentificationofsinglemoleculesinaqueoussolution |