Nitrogen content variation in archaeological bone and its implications for stable isotope analysis and radiocarbon dating
The collagen component of ancient bones is routinely isolated for radiocarbon dating and stable isotope studies. However, it is impossible to tell the state of collagen preservation from visual inspection of bones. At the Oxford Radiocarbon Accelerator Unit (ORAU), the percent nitrogen by weight (%N...
| Main Authors: | , , , , , |
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| Format: | Journal article |
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Elsevier
2018
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| _version_ | 1797055415236689920 |
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| author | Jacob, E Querci, D Caparros, M Barroso Ruiz, C Higham, T Deviese, T |
| author_facet | Jacob, E Querci, D Caparros, M Barroso Ruiz, C Higham, T Deviese, T |
| author_sort | Jacob, E |
| collection | OXFORD |
| description | The collagen component of ancient bones is routinely isolated for radiocarbon dating and stable isotope studies. However, it is impossible to tell the state of collagen preservation from visual inspection of bones. At the Oxford Radiocarbon Accelerator Unit (ORAU), the percent nitrogen by weight (%N) of a ∼5 mg sample of bone powder is measured on a mass spectrometer and used as a proxy for protein content. A previous study showed that samples with %N > 0.76 are considered likely to produce sufficient collagen for radiocarbon dating (Brock et al., 2010b). However, the extent of variation between bone %N and collagen yield is unclear, as is the intra-bone variation in %N. Here, we report a series of tests performed on Palaeolithic bones known to have variable collagen preservation. This new study shows significant variation in %N within the same bone and that there is sometimes a lack of correlation between %N and collagen yield. These results suggest that for bone samples from difficult environments or from Pleistocene contexts, it may be worth sub-sampling for %N in different locations of the bone (if possible) and then attempting to extract collagen from marginally preserved bones (%N around 0.2–0.7%), as they may still yield sufficient collagen for isotope and dating studies. |
| first_indexed | 2024-03-06T19:10:23Z |
| format | Journal article |
| id | oxford-uuid:1693f676-73a6-45ba-b4fc-c289b0b12191 |
| institution | University of Oxford |
| last_indexed | 2024-03-06T19:10:23Z |
| publishDate | 2018 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | oxford-uuid:1693f676-73a6-45ba-b4fc-c289b0b121912022-03-26T10:32:07ZNitrogen content variation in archaeological bone and its implications for stable isotope analysis and radiocarbon datingJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:1693f676-73a6-45ba-b4fc-c289b0b12191Symplectic Elements at OxfordElsevier2018Jacob, EQuerci, DCaparros, MBarroso Ruiz, CHigham, TDeviese, TThe collagen component of ancient bones is routinely isolated for radiocarbon dating and stable isotope studies. However, it is impossible to tell the state of collagen preservation from visual inspection of bones. At the Oxford Radiocarbon Accelerator Unit (ORAU), the percent nitrogen by weight (%N) of a ∼5 mg sample of bone powder is measured on a mass spectrometer and used as a proxy for protein content. A previous study showed that samples with %N > 0.76 are considered likely to produce sufficient collagen for radiocarbon dating (Brock et al., 2010b). However, the extent of variation between bone %N and collagen yield is unclear, as is the intra-bone variation in %N. Here, we report a series of tests performed on Palaeolithic bones known to have variable collagen preservation. This new study shows significant variation in %N within the same bone and that there is sometimes a lack of correlation between %N and collagen yield. These results suggest that for bone samples from difficult environments or from Pleistocene contexts, it may be worth sub-sampling for %N in different locations of the bone (if possible) and then attempting to extract collagen from marginally preserved bones (%N around 0.2–0.7%), as they may still yield sufficient collagen for isotope and dating studies. |
| spellingShingle | Jacob, E Querci, D Caparros, M Barroso Ruiz, C Higham, T Deviese, T Nitrogen content variation in archaeological bone and its implications for stable isotope analysis and radiocarbon dating |
| title | Nitrogen content variation in archaeological bone and its implications for stable isotope analysis and radiocarbon dating |
| title_full | Nitrogen content variation in archaeological bone and its implications for stable isotope analysis and radiocarbon dating |
| title_fullStr | Nitrogen content variation in archaeological bone and its implications for stable isotope analysis and radiocarbon dating |
| title_full_unstemmed | Nitrogen content variation in archaeological bone and its implications for stable isotope analysis and radiocarbon dating |
| title_short | Nitrogen content variation in archaeological bone and its implications for stable isotope analysis and radiocarbon dating |
| title_sort | nitrogen content variation in archaeological bone and its implications for stable isotope analysis and radiocarbon dating |
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