The ratio between collagenase class I and class II influences the efficient islet release from the rat pancreas.

The ratio between collagenase class I and class II influences the efficient islet release from the rat pancreas.

BACKGROUND: Previous studies indicated different roles of collagenase class I, class II and neutral protease in the enzymatic islet release from pancreatic tissue. Because no information has been available, this study was aimed to investigate the isolation efficiency of different ratios between col...

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Bibliografiske detaljer
Main Authors:	Brandhorst, H, Raemsch-Guenther, N, Raemsch, C, Friedrich, O, Huettler, S, Kurfuerst, M, Korsgren, O, Brandhorst, D
Format:	Journal article
Sprog:	English
Udgivet:	2008

Lignende værker

The ratio between class II and class I collagenase determines the amount of neutral protease activity required for efficient islet release from the rat pancreas.
af: Brandhorst, H, et al.
Udgivet: (2005)

Adjustment of the ratio between collagenase class II and I improves islet isolation outcome.
af: Brandhorst, D, et al.
Udgivet: (2005)

Degraded collagenase deteriorates islet viability.
af: Brandhorst, H, et al.
Udgivet: (2008)

Comparison of neutral proteases and collagenase class I as essential enzymes for human islet isolation
af: Brandhorst, H, et al.
Udgivet: (2015)

The effect of truncated collagenase class I isomers on human islet isolation outcome.
af: Brandhorst, H, et al.
Udgivet: (2010)

Pig Pancreas Oxygenation at 20°C Increases Islet ATP Generation but Deteriorates Islet Function
af: M. Iken, et al.
Udgivet: (2009-07-01)

Pig pancreas oxygenation at 20 degrees C increases islet ATP generation but deteriorates islet function.
af: Iken, M, et al.
Udgivet: (2009)

New class of oxygen carriers improves islet isolation from long-term stored rat pancreata.
af: Brandhorst, H, et al.
Udgivet: (2008)

Influence of collagenase loading on long-term preservation of pig pancreas by the two-layer method for subsequent islet isolation.
af: Brandhorst, D, et al.
Udgivet: (2005)

Successful human islet isolation utilizing recombinant collagenase.
af: Brandhorst, H, et al.
Udgivet: (2003)

Large-Scale Comparison of Liberase HI and Collagenase NB1 Utilized for Human Islet Isolation
af: H. Brandhorst, et al.
Udgivet: (2010-01-01)

Vitacyte collagenase HA: a novel enzyme blend for efficient human islet isolation.
af: Caballero-Corbalán, J, et al.
Udgivet: (2009)

Large-scale comparison of Liberase HI and collagenase NB1 utilized for human islet isolation.
af: Brandhorst, H, et al.
Udgivet: (2010)

Quality of isolated pig islets is improved using perfluorohexyloctane for pancreas storage in a split lobe model.
af: Brandhorst, H, et al.
Udgivet: (2013)

Quality of Isolated Pig Islets is Improved Using Perfluorohexyloctane for Pancreas Storage in a Split Lobe Model
af: Brandhorst, H, et al.
Udgivet: (2013)

Using HTK for prolonged pancreas preservation prior to human islet isolation
af: Caballero-Corbalán, J, et al.
Udgivet: (2012)

Using HTK for prolonged pancreas preservation prior to human islet isolation.
af: Caballero-Corbalán, J, et al.
Udgivet: (2012)

Multicenter assessment of animal-free collagenase AF-1 for human islet isolation
af: Brandhorst, D, et al.
Udgivet: (2017)

Quantifying the effects of different neutral proteases on human islet integrity
af: Brandhorst, H, et al.
Udgivet: (2018)

Quality of Isolated Pig Islets is Improved Using Perfluorohexyloctane for Pancreas Storage in a Split Lobe Model
af: H. Brandhorst, et al.
Udgivet: (2013-08-01)

Perfluorohexyloctane improves long-term storage of rat pancreata for subsequent islet isolation.
af: Brandhorst, H, et al.
Udgivet: (2009)

Human islet separation utilizing a closed automated purification system.
af: Friberg, A, et al.
Udgivet: (2008)

Human Islet Separation Utilizing a Closed Automated Purification System
af: A. S. Friberg, et al.
Udgivet: (2008-12-01)

Multicenter Assessment of Animal-free Collagenase AF-1 for Human Islet Isolation
af: Daniel Brandhorst, et al.
Udgivet: (2017-10-01)

Quantifying the Effects of Different Neutral Proteases on Human Islet Integrity
af: Heide Brandhorst, et al.
Udgivet: (2017-11-01)

Comparison of Clostripain and Neutral Protease as Supplementary Enzymes for Human Islet Isolation
af: Brandhorst, H, et al.
Udgivet: (2018)

Long-term preservation of the pig pancreas by a one-layer method for successful islet isolation.
af: Brandhorst, D, et al.
Udgivet: (2005)

Evaluation of perfluorohexyloctane/polydimethylsiloxane for pancreas preservation for clinical islet isolation and transplantation
af: Sthle, M, et al.
Udgivet: (2016)

Successful pancreas preservation by a perfluorocarbon-based one-layer method for subsequent pig islet isolation.
af: Brandhorst, D, et al.
Udgivet: (2005)

Localisation of collagenase within islets following infusion into human pancreas
af: Cross, S, et al.
Udgivet: (2007)

Pancreas storage in oxygenated perfluorodecalin does not restore post-transplant function of isolated pig islets pre-damaged by warm ischemia.
af: Brandhorst, D, et al.
Udgivet: (2006)

Erratum: Vitacyte collagenase HA: A novel enzyme blend for efficient human islet isolation (Transplantation (2009) 88 (1400))
af: Caballero-Corbalán, J, et al.
Udgivet: (2010)

Adaption of neutral protease activity for islet isolation from the long-term two-layer method-stored pig pancreas.
af: Brandhorst, D, et al.
Udgivet: (2005)

Evaluation of Perfluorohexyloctane/Polydimethylsiloxane for Pancreas Preservation for Clinical Islet Isolation and Transplantation
af: Magnus Ståhle, et al.
Udgivet: (2016-12-01)

Comparison of Clostripain and Neutral Protease as Supplementary Enzymes for Human Islet Isolation
af: Heide Brandhorst, et al.
Udgivet: (2019-02-01)

Pancreatic islets: methods for isolation and purification of juvenile and adult pig islets
af: Brandhorst, H, et al.
Udgivet: (2016)

Pathogen inactivation of human serum facilitates its clinical use for islet cell culture and subsequent transplantation.
af: Ståhle, M, et al.
Udgivet: (2011)

Quantification of the islet product: presentation of a standardized current good manufacturing practices compliant system with minimal variability.
af: Friberg, A, et al.
Udgivet: (2011)

Long-term engraftment and function of transplanted pancreatic islets in vascularized segments of small intestine.
af: Kakabadze, Z, et al.
Udgivet: (2011)

Prevention of early islet graft failure by selective inducible nitric oxide synthase inhibitors after pig to nude rat intraportal islet transplantation.
af: Brandhorst, D, et al.
Udgivet: (2001)