Species-specific transcription in mice carrying human chromosome 21.
Homologous sets of transcription factors direct conserved tissue-specific gene expression, yet transcription factor-binding events diverge rapidly between closely related species. We used hepatocytes from an aneuploid mouse strain carrying human chromosome 21 to determine, on a chromosomal scale, wh...
| Main Authors: | , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2008
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| _version_ | 1797055496313634816 |
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| author | Wilson, MD Barbosa-Morais, N Schmidt, D Conboy, C Vanes, L Tybulewicz, V Fisher, E Tavaré, S Odom, D |
| author_facet | Wilson, MD Barbosa-Morais, N Schmidt, D Conboy, C Vanes, L Tybulewicz, V Fisher, E Tavaré, S Odom, D |
| author_sort | Wilson, MD |
| collection | OXFORD |
| description | Homologous sets of transcription factors direct conserved tissue-specific gene expression, yet transcription factor-binding events diverge rapidly between closely related species. We used hepatocytes from an aneuploid mouse strain carrying human chromosome 21 to determine, on a chromosomal scale, whether interspecies differences in transcriptional regulation are primarily directed by human genetic sequence or mouse nuclear environment. Virtually all transcription factor-binding locations, landmarks of transcription initiation, and the resulting gene expression observed in human hepatocytes were recapitulated across the entire human chromosome 21 in the mouse hepatocyte nucleus. Thus, in homologous tissues, genetic sequence is largely responsible for directing transcriptional programs; interspecies differences in epigenetic machinery, cellular environment, and transcription factors themselves play secondary roles. |
| first_indexed | 2024-03-06T19:11:30Z |
| format | Journal article |
| id | oxford-uuid:16ee61a2-6fef-4cfb-bf63-3e70902cf42d |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T19:11:30Z |
| publishDate | 2008 |
| record_format | dspace |
| spelling | oxford-uuid:16ee61a2-6fef-4cfb-bf63-3e70902cf42d2022-03-26T10:34:08ZSpecies-specific transcription in mice carrying human chromosome 21.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:16ee61a2-6fef-4cfb-bf63-3e70902cf42dEnglishSymplectic Elements at Oxford2008Wilson, MDBarbosa-Morais, NSchmidt, DConboy, CVanes, LTybulewicz, VFisher, ETavaré, SOdom, DHomologous sets of transcription factors direct conserved tissue-specific gene expression, yet transcription factor-binding events diverge rapidly between closely related species. We used hepatocytes from an aneuploid mouse strain carrying human chromosome 21 to determine, on a chromosomal scale, whether interspecies differences in transcriptional regulation are primarily directed by human genetic sequence or mouse nuclear environment. Virtually all transcription factor-binding locations, landmarks of transcription initiation, and the resulting gene expression observed in human hepatocytes were recapitulated across the entire human chromosome 21 in the mouse hepatocyte nucleus. Thus, in homologous tissues, genetic sequence is largely responsible for directing transcriptional programs; interspecies differences in epigenetic machinery, cellular environment, and transcription factors themselves play secondary roles. |
| spellingShingle | Wilson, MD Barbosa-Morais, N Schmidt, D Conboy, C Vanes, L Tybulewicz, V Fisher, E Tavaré, S Odom, D Species-specific transcription in mice carrying human chromosome 21. |
| title | Species-specific transcription in mice carrying human chromosome 21. |
| title_full | Species-specific transcription in mice carrying human chromosome 21. |
| title_fullStr | Species-specific transcription in mice carrying human chromosome 21. |
| title_full_unstemmed | Species-specific transcription in mice carrying human chromosome 21. |
| title_short | Species-specific transcription in mice carrying human chromosome 21. |
| title_sort | species specific transcription in mice carrying human chromosome 21 |
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