Ha-ras hypervariable alleles in myelodysplasia.
The somatic mutation of one of the ras oncogenes is now considered to be a critical step in the pathogenesis of many tumours. Circumstantial evidence also suggests that some individuals may be genetically predisposed to malignancy and a general method used to analyse such disease susceptibility is t...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
1986
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| _version_ | 1797055727146106880 |
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| author | Thein, S Oscier, D Flint, J Wainscoat, J |
| author_facet | Thein, S Oscier, D Flint, J Wainscoat, J |
| author_sort | Thein, S |
| collection | OXFORD |
| description | The somatic mutation of one of the ras oncogenes is now considered to be a critical step in the pathogenesis of many tumours. Circumstantial evidence also suggests that some individuals may be genetically predisposed to malignancy and a general method used to analyse such disease susceptibility is the study of restriction fragment length polymorphisms (RFLPs) at particular loci. The Harvey ras (Ha-ras) locus includes a hypervariable region (HVR) which consists of a series of 28-base-pair (bp) tandem repeats 3' to the gene. This arrangement gives rise to alleles of a wide range of sizes, making such genetic analysis possible. A previous study reported that white blood cell DNA from cancer patients frequently showed allelic restriction fragments at the Ha-ras locus which were found only rarely in normal unaffected individuals, and it was concluded that the inheritance of such unusual alleles may be linked to a susceptibility to cancer. As this conclusion has major implications we sought to investigate whether this association could be confirmed in patients with myelodysplasia, a common haematological malignancy reported to have the highest frequency of rare alleles. The Ha-ras alleles were characterized in normal healthy individuals and compared with those found in patients with myelodysplasia (MDS). Our results, reported here, show that the distribution of Ha-ras alleles in myelodysplastic patients is not significantly different from that in normal individuals. |
| first_indexed | 2024-03-06T19:13:55Z |
| format | Journal article |
| id | oxford-uuid:17b3f338-6e26-47e5-8b7d-4ea48afbba6c |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T19:13:55Z |
| publishDate | 1986 |
| record_format | dspace |
| spelling | oxford-uuid:17b3f338-6e26-47e5-8b7d-4ea48afbba6c2022-03-26T10:38:57ZHa-ras hypervariable alleles in myelodysplasia.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:17b3f338-6e26-47e5-8b7d-4ea48afbba6cEnglishSymplectic Elements at Oxford1986Thein, SOscier, DFlint, JWainscoat, JThe somatic mutation of one of the ras oncogenes is now considered to be a critical step in the pathogenesis of many tumours. Circumstantial evidence also suggests that some individuals may be genetically predisposed to malignancy and a general method used to analyse such disease susceptibility is the study of restriction fragment length polymorphisms (RFLPs) at particular loci. The Harvey ras (Ha-ras) locus includes a hypervariable region (HVR) which consists of a series of 28-base-pair (bp) tandem repeats 3' to the gene. This arrangement gives rise to alleles of a wide range of sizes, making such genetic analysis possible. A previous study reported that white blood cell DNA from cancer patients frequently showed allelic restriction fragments at the Ha-ras locus which were found only rarely in normal unaffected individuals, and it was concluded that the inheritance of such unusual alleles may be linked to a susceptibility to cancer. As this conclusion has major implications we sought to investigate whether this association could be confirmed in patients with myelodysplasia, a common haematological malignancy reported to have the highest frequency of rare alleles. The Ha-ras alleles were characterized in normal healthy individuals and compared with those found in patients with myelodysplasia (MDS). Our results, reported here, show that the distribution of Ha-ras alleles in myelodysplastic patients is not significantly different from that in normal individuals. |
| spellingShingle | Thein, S Oscier, D Flint, J Wainscoat, J Ha-ras hypervariable alleles in myelodysplasia. |
| title | Ha-ras hypervariable alleles in myelodysplasia. |
| title_full | Ha-ras hypervariable alleles in myelodysplasia. |
| title_fullStr | Ha-ras hypervariable alleles in myelodysplasia. |
| title_full_unstemmed | Ha-ras hypervariable alleles in myelodysplasia. |
| title_short | Ha-ras hypervariable alleles in myelodysplasia. |
| title_sort | ha ras hypervariable alleles in myelodysplasia |
| work_keys_str_mv | AT theins harashypervariableallelesinmyelodysplasia AT oscierd harashypervariableallelesinmyelodysplasia AT flintj harashypervariableallelesinmyelodysplasia AT wainscoatj harashypervariableallelesinmyelodysplasia |