miR-10b-5p is a novel Th17 regulator present in Th17 cells from Ankylosing Spondylitis
<p>Objective: to determine the microRNA (miR) signature in ankylosing spondylitis (AS) Th17 cells.</p><p>Methods: IL-17A-producing CD4+ T cells from AS patients and healthy controls were FACS-sorted for miR RNA sequencing and qPCR validation. miR-10b function was determined by miR...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Journal article |
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BMJ Publishing Group
2017
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| _version_ | 1797055772640673792 |
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| author | Chen, L Al-Mossawi, M Ridley, A Sekine, T Hammitzsch, A de Wit, J Shi, H Penkava, F Stolarska, M Pulyakhina, I Knight, J Kim, T Bowness, P |
| author_facet | Chen, L Al-Mossawi, M Ridley, A Sekine, T Hammitzsch, A de Wit, J Shi, H Penkava, F Stolarska, M Pulyakhina, I Knight, J Kim, T Bowness, P |
| author_sort | Chen, L |
| collection | OXFORD |
| description | <p>Objective: to determine the microRNA (miR) signature in ankylosing spondylitis (AS) Th17 cells.</p><p>Methods: IL-17A-producing CD4+ T cells from AS patients and healthy controls were FACS-sorted for miR RNA sequencing and qPCR validation. miR-10b function was determined by miR mimic expression followed by cytokine measurement, transcriptome analysis, qPCR and luciferase assays.</p> <p>Results: AS Th17 cells exhibited a miR signature characterized by upregulation of miR-155-5p, miR- 210-3p and miR-10b. miR-10b has not been described previously in Th17 cells and was selected for further characterization. miR-10b is transiently induced in in-vitro differentiated Th17 cells. Transcriptome, qPCR and luciferase assays suggest that MAP3K7 is targeted by miR-10b. Both miR-10b over-expression and MAP3K7 silencing inhibited production of IL-17A by both total CD4 and differentiating Th17 cells.</p> <p>Conclusions: AS Th17 cells have a specific miR signature and upregulate miR-10b in vitro. Our data suggest that miR-10b is upregulated by pro-inflammatory cytokines and may act as a feedback loop to suppress IL-17A by targeting MAP3K7. miR-10b is a potential therapeutic candidate to suppress pathogenic Th17 cell function in AS patients.</p> |
| first_indexed | 2024-03-06T19:14:34Z |
| format | Journal article |
| id | oxford-uuid:17e71ab5-8629-4720-85a0-bc360ec01f06 |
| institution | University of Oxford |
| last_indexed | 2024-03-06T19:14:34Z |
| publishDate | 2017 |
| publisher | BMJ Publishing Group |
| record_format | dspace |
| spelling | oxford-uuid:17e71ab5-8629-4720-85a0-bc360ec01f062022-03-26T10:40:22ZmiR-10b-5p is a novel Th17 regulator present in Th17 cells from Ankylosing SpondylitisJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:17e71ab5-8629-4720-85a0-bc360ec01f06Symplectic Elements at OxfordBMJ Publishing Group2017Chen, LAl-Mossawi, MRidley, ASekine, THammitzsch, Ade Wit, JShi, HPenkava, FStolarska, MPulyakhina, IKnight, JKim, TBowness, P<p>Objective: to determine the microRNA (miR) signature in ankylosing spondylitis (AS) Th17 cells.</p><p>Methods: IL-17A-producing CD4+ T cells from AS patients and healthy controls were FACS-sorted for miR RNA sequencing and qPCR validation. miR-10b function was determined by miR mimic expression followed by cytokine measurement, transcriptome analysis, qPCR and luciferase assays.</p> <p>Results: AS Th17 cells exhibited a miR signature characterized by upregulation of miR-155-5p, miR- 210-3p and miR-10b. miR-10b has not been described previously in Th17 cells and was selected for further characterization. miR-10b is transiently induced in in-vitro differentiated Th17 cells. Transcriptome, qPCR and luciferase assays suggest that MAP3K7 is targeted by miR-10b. Both miR-10b over-expression and MAP3K7 silencing inhibited production of IL-17A by both total CD4 and differentiating Th17 cells.</p> <p>Conclusions: AS Th17 cells have a specific miR signature and upregulate miR-10b in vitro. Our data suggest that miR-10b is upregulated by pro-inflammatory cytokines and may act as a feedback loop to suppress IL-17A by targeting MAP3K7. miR-10b is a potential therapeutic candidate to suppress pathogenic Th17 cell function in AS patients.</p> |
| spellingShingle | Chen, L Al-Mossawi, M Ridley, A Sekine, T Hammitzsch, A de Wit, J Shi, H Penkava, F Stolarska, M Pulyakhina, I Knight, J Kim, T Bowness, P miR-10b-5p is a novel Th17 regulator present in Th17 cells from Ankylosing Spondylitis |
| title | miR-10b-5p is a novel Th17 regulator present in Th17 cells from Ankylosing Spondylitis |
| title_full | miR-10b-5p is a novel Th17 regulator present in Th17 cells from Ankylosing Spondylitis |
| title_fullStr | miR-10b-5p is a novel Th17 regulator present in Th17 cells from Ankylosing Spondylitis |
| title_full_unstemmed | miR-10b-5p is a novel Th17 regulator present in Th17 cells from Ankylosing Spondylitis |
| title_short | miR-10b-5p is a novel Th17 regulator present in Th17 cells from Ankylosing Spondylitis |
| title_sort | mir 10b 5p is a novel th17 regulator present in th17 cells from ankylosing spondylitis |
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