The crystal structure of d(G-G-G-G-C-C-C-C). A model for poly(dG).poly(dC).
The structure of the DNA oligomer d(G-G-G-G-C-C-C-C) has been determined at a resolution of 2.5 A by single-crystal X-ray methods. There are two strands in the asymmetric unit, and these coil about each other to form a right-handed double-helix of the A-type with Watson-Crick hydrogen bonds between...
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| Format: | Journal article |
| Language: | English |
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1985
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| _version_ | 1826261041240080384 |
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| author | McCall, M Brown, T Kennard, O |
| author_facet | McCall, M Brown, T Kennard, O |
| author_sort | McCall, M |
| collection | OXFORD |
| description | The structure of the DNA oligomer d(G-G-G-G-C-C-C-C) has been determined at a resolution of 2.5 A by single-crystal X-ray methods. There are two strands in the asymmetric unit, and these coil about each other to form a right-handed double-helix of the A-type with Watson-Crick hydrogen bonds between base-pairs. The helix has a shallow minor groove and a deep, water-filled major groove; almost all exposed functional groups on the DNA are hydrated, and 106 ordered solvent molecules have been found. The two d(G-G-G-G).d(C-C-C-C) segments in the octamer exhibit similar and uniform structures, but there is a slight discontinuity at the GpC step between them. A recurring feature of the structure is the overlap of adjacent guanine bases in each GpG step, with the five-membered ring of one guanine stacking on the six-membered ring of its neighbour. There is little or no overlap between adjacent cytosine rings. Conformational parameters for these GpG steps are compared with those from other single-crystal X-ray analyses. In general, GpG steps exhibit high slide, low roll and variable twist. Models for poly(dG).poly(dC) were generated by applying a simple rotation and translation to each of the unmodified d(G-G-G-G).d(C-C-C-C) units. Detailed features of these models are shown to be compatible with various assays of poly(dG).poly(dC) in solution, and are useful in understanding the polymorphic behaviour of this sequence under a variety of experimental conditions. |
| first_indexed | 2024-03-06T19:15:18Z |
| format | Journal article |
| id | oxford-uuid:18245676-2785-4295-925e-29a0a810aa47 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T19:15:18Z |
| publishDate | 1985 |
| record_format | dspace |
| spelling | oxford-uuid:18245676-2785-4295-925e-29a0a810aa472022-03-26T10:41:37ZThe crystal structure of d(G-G-G-G-C-C-C-C). A model for poly(dG).poly(dC).Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:18245676-2785-4295-925e-29a0a810aa47EnglishSymplectic Elements at Oxford1985McCall, MBrown, TKennard, OThe structure of the DNA oligomer d(G-G-G-G-C-C-C-C) has been determined at a resolution of 2.5 A by single-crystal X-ray methods. There are two strands in the asymmetric unit, and these coil about each other to form a right-handed double-helix of the A-type with Watson-Crick hydrogen bonds between base-pairs. The helix has a shallow minor groove and a deep, water-filled major groove; almost all exposed functional groups on the DNA are hydrated, and 106 ordered solvent molecules have been found. The two d(G-G-G-G).d(C-C-C-C) segments in the octamer exhibit similar and uniform structures, but there is a slight discontinuity at the GpC step between them. A recurring feature of the structure is the overlap of adjacent guanine bases in each GpG step, with the five-membered ring of one guanine stacking on the six-membered ring of its neighbour. There is little or no overlap between adjacent cytosine rings. Conformational parameters for these GpG steps are compared with those from other single-crystal X-ray analyses. In general, GpG steps exhibit high slide, low roll and variable twist. Models for poly(dG).poly(dC) were generated by applying a simple rotation and translation to each of the unmodified d(G-G-G-G).d(C-C-C-C) units. Detailed features of these models are shown to be compatible with various assays of poly(dG).poly(dC) in solution, and are useful in understanding the polymorphic behaviour of this sequence under a variety of experimental conditions. |
| spellingShingle | McCall, M Brown, T Kennard, O The crystal structure of d(G-G-G-G-C-C-C-C). A model for poly(dG).poly(dC). |
| title | The crystal structure of d(G-G-G-G-C-C-C-C). A model for poly(dG).poly(dC). |
| title_full | The crystal structure of d(G-G-G-G-C-C-C-C). A model for poly(dG).poly(dC). |
| title_fullStr | The crystal structure of d(G-G-G-G-C-C-C-C). A model for poly(dG).poly(dC). |
| title_full_unstemmed | The crystal structure of d(G-G-G-G-C-C-C-C). A model for poly(dG).poly(dC). |
| title_short | The crystal structure of d(G-G-G-G-C-C-C-C). A model for poly(dG).poly(dC). |
| title_sort | crystal structure of d g g g g c c c c a model for poly dg poly dc |
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