Intercellular adhesion molecule-1 is an endothelial cell adhesion receptor for Plasmodium falciparum.
The primary event in the pathogenesis of severe malaria in Plasmodium falciparum infection is thought to be adherence of trophozoite- and schizont-infected erythrocytes to capillary endothelium, a process called sequestration. Identifying the endothelial molecules used as receptors is an essential s...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
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1989
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| _version_ | 1797056044852051968 |
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| author | Berendt, A Simmons, D Tansey, J Newbold, C Marsh, K |
| author_facet | Berendt, A Simmons, D Tansey, J Newbold, C Marsh, K |
| author_sort | Berendt, A |
| collection | OXFORD |
| description | The primary event in the pathogenesis of severe malaria in Plasmodium falciparum infection is thought to be adherence of trophozoite- and schizont-infected erythrocytes to capillary endothelium, a process called sequestration. Identifying the endothelial molecules used as receptors is an essential step in understanding this disease process. Recent work implicates the membrane glycoprotein CD36 (platelet glycoprotein IV; refs 2-5) and the multi-functional glycoprotein thrombospondin as receptors. Although CD36 has a widespread distribution on microvascular endothelium, it may not be expressed on all capillary beds where sequestration occurs, especially in the brain. The role of thrombospondin in cell adhesion, in vitro or in vivo, is less certain. We have noticed that some parasites bind to human umbilical-vein endothelial cells independently of CD36 or thrombospondin. To screen for alternative receptors, we have developed a novel cell-adhesion assay using transfected COS cells, which confirms that CD36 is a cell-adhesion receptor. In addition, we find that an endothelial-binding line of P. falciparum binds to COS cells transfected with a complementary DNA encoding intercellular adhesion molecule-1. As this molecule is widely distributed on capillaries and is inducible, this finding may be relevant to the pathogenesis of severe malaria. |
| first_indexed | 2024-03-06T19:17:47Z |
| format | Journal article |
| id | oxford-uuid:19031b2e-727f-4963-8316-b90fdd78b0fd |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T19:17:47Z |
| publishDate | 1989 |
| record_format | dspace |
| spelling | oxford-uuid:19031b2e-727f-4963-8316-b90fdd78b0fd2022-03-26T10:46:29ZIntercellular adhesion molecule-1 is an endothelial cell adhesion receptor for Plasmodium falciparum.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:19031b2e-727f-4963-8316-b90fdd78b0fdEnglishSymplectic Elements at Oxford1989Berendt, ASimmons, DTansey, JNewbold, CMarsh, KThe primary event in the pathogenesis of severe malaria in Plasmodium falciparum infection is thought to be adherence of trophozoite- and schizont-infected erythrocytes to capillary endothelium, a process called sequestration. Identifying the endothelial molecules used as receptors is an essential step in understanding this disease process. Recent work implicates the membrane glycoprotein CD36 (platelet glycoprotein IV; refs 2-5) and the multi-functional glycoprotein thrombospondin as receptors. Although CD36 has a widespread distribution on microvascular endothelium, it may not be expressed on all capillary beds where sequestration occurs, especially in the brain. The role of thrombospondin in cell adhesion, in vitro or in vivo, is less certain. We have noticed that some parasites bind to human umbilical-vein endothelial cells independently of CD36 or thrombospondin. To screen for alternative receptors, we have developed a novel cell-adhesion assay using transfected COS cells, which confirms that CD36 is a cell-adhesion receptor. In addition, we find that an endothelial-binding line of P. falciparum binds to COS cells transfected with a complementary DNA encoding intercellular adhesion molecule-1. As this molecule is widely distributed on capillaries and is inducible, this finding may be relevant to the pathogenesis of severe malaria. |
| spellingShingle | Berendt, A Simmons, D Tansey, J Newbold, C Marsh, K Intercellular adhesion molecule-1 is an endothelial cell adhesion receptor for Plasmodium falciparum. |
| title | Intercellular adhesion molecule-1 is an endothelial cell adhesion receptor for Plasmodium falciparum. |
| title_full | Intercellular adhesion molecule-1 is an endothelial cell adhesion receptor for Plasmodium falciparum. |
| title_fullStr | Intercellular adhesion molecule-1 is an endothelial cell adhesion receptor for Plasmodium falciparum. |
| title_full_unstemmed | Intercellular adhesion molecule-1 is an endothelial cell adhesion receptor for Plasmodium falciparum. |
| title_short | Intercellular adhesion molecule-1 is an endothelial cell adhesion receptor for Plasmodium falciparum. |
| title_sort | intercellular adhesion molecule 1 is an endothelial cell adhesion receptor for plasmodium falciparum |
| work_keys_str_mv | AT berendta intercellularadhesionmolecule1isanendothelialcelladhesionreceptorforplasmodiumfalciparum AT simmonsd intercellularadhesionmolecule1isanendothelialcelladhesionreceptorforplasmodiumfalciparum AT tanseyj intercellularadhesionmolecule1isanendothelialcelladhesionreceptorforplasmodiumfalciparum AT newboldc intercellularadhesionmolecule1isanendothelialcelladhesionreceptorforplasmodiumfalciparum AT marshk intercellularadhesionmolecule1isanendothelialcelladhesionreceptorforplasmodiumfalciparum |