Characterization of a monoclonal antibody that neutralizes the hyaluronidase activity of Russell's viper venom.
Three monoclonal antibodies (WPN1, WPN2 and WPN3) raised against a partially purified fraction of Russell's viper venom (RVV) were characterized. All three monoclonal antibodies reacted with crude RVV when tested by ELISA, but only two (WPN1, WPN2) neutralized its hyaluronidase activity. WPN1 w...
| Main Authors: | , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
1990
|
| _version_ | 1826261202405163008 |
|---|---|
| author | Pukrittayakamee, S Nontprasert, A White, N Warrell, D Bunnag, D |
| author_facet | Pukrittayakamee, S Nontprasert, A White, N Warrell, D Bunnag, D |
| author_sort | Pukrittayakamee, S |
| collection | OXFORD |
| description | Three monoclonal antibodies (WPN1, WPN2 and WPN3) raised against a partially purified fraction of Russell's viper venom (RVV) were characterized. All three monoclonal antibodies reacted with crude RVV when tested by ELISA, but only two (WPN1, WPN2) neutralized its hyaluronidase activity. WPN1 was the more potent and was effective at an antigen: antibody ratio of 1:3. Furthermore, WPN1 was shown to recognize only the 14,000 MW component of crude RVV. This has been identified in a previous study to be hyaluronidase. This antibody was also found to recognize some components of Calloselasma rhodostoma venom which also possesses potent hyaluronidase activity. The potential therapeutic role of antibodies that neutralize the hyaluronidase component of snake venoms should be investigated further. |
| first_indexed | 2024-03-06T19:17:49Z |
| format | Journal article |
| id | oxford-uuid:1905c14e-de32-4237-abca-8473963eddaf |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T19:17:49Z |
| publishDate | 1990 |
| record_format | dspace |
| spelling | oxford-uuid:1905c14e-de32-4237-abca-8473963eddaf2022-03-26T10:46:30ZCharacterization of a monoclonal antibody that neutralizes the hyaluronidase activity of Russell's viper venom.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:1905c14e-de32-4237-abca-8473963eddafEnglishSymplectic Elements at Oxford1990Pukrittayakamee, SNontprasert, AWhite, NWarrell, DBunnag, DThree monoclonal antibodies (WPN1, WPN2 and WPN3) raised against a partially purified fraction of Russell's viper venom (RVV) were characterized. All three monoclonal antibodies reacted with crude RVV when tested by ELISA, but only two (WPN1, WPN2) neutralized its hyaluronidase activity. WPN1 was the more potent and was effective at an antigen: antibody ratio of 1:3. Furthermore, WPN1 was shown to recognize only the 14,000 MW component of crude RVV. This has been identified in a previous study to be hyaluronidase. This antibody was also found to recognize some components of Calloselasma rhodostoma venom which also possesses potent hyaluronidase activity. The potential therapeutic role of antibodies that neutralize the hyaluronidase component of snake venoms should be investigated further. |
| spellingShingle | Pukrittayakamee, S Nontprasert, A White, N Warrell, D Bunnag, D Characterization of a monoclonal antibody that neutralizes the hyaluronidase activity of Russell's viper venom. |
| title | Characterization of a monoclonal antibody that neutralizes the hyaluronidase activity of Russell's viper venom. |
| title_full | Characterization of a monoclonal antibody that neutralizes the hyaluronidase activity of Russell's viper venom. |
| title_fullStr | Characterization of a monoclonal antibody that neutralizes the hyaluronidase activity of Russell's viper venom. |
| title_full_unstemmed | Characterization of a monoclonal antibody that neutralizes the hyaluronidase activity of Russell's viper venom. |
| title_short | Characterization of a monoclonal antibody that neutralizes the hyaluronidase activity of Russell's viper venom. |
| title_sort | characterization of a monoclonal antibody that neutralizes the hyaluronidase activity of russell s viper venom |
| work_keys_str_mv | AT pukrittayakamees characterizationofamonoclonalantibodythatneutralizesthehyaluronidaseactivityofrussellsvipervenom AT nontpraserta characterizationofamonoclonalantibodythatneutralizesthehyaluronidaseactivityofrussellsvipervenom AT whiten characterizationofamonoclonalantibodythatneutralizesthehyaluronidaseactivityofrussellsvipervenom AT warrelld characterizationofamonoclonalantibodythatneutralizesthehyaluronidaseactivityofrussellsvipervenom AT bunnagd characterizationofamonoclonalantibodythatneutralizesthehyaluronidaseactivityofrussellsvipervenom |