Droplet-on-hydrogel bilayer based assay for functional study of membrane proteins

Droplet-on-hydrogel bilayer based assay for functional study of membrane proteins

<p>Functional study of membrane proteins, especially when a transmembrane potential is required for protein function, is notoriously difficult. In this thesis, I introduce an experimental assay that adapts the droplet-on-hydrogel technique for a high-resolution functional study of membrane pro...

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Bibliografiske detaljer
Hovedforfatter:	Cocul’ová, Z
Andre forfattere:	Berry, R
Format:	Thesis
Sprog:	English
Udgivet:	2022
Fag:	Biophysics

Lignende værker

Applications of droplet interface bilayers: specific capacitance measurements and membrane protein corralling
af: Gross, L, et al.
Udgivet: (2011)

Combining single-molecule fluorescence and single-channel recording in droplet interface bilayers
af: weatherill, E
Udgivet: (2015)

Droplet interface bilayers for the study of membrane proteins
af: Hwang, W
Udgivet: (2008)

Imaging lipid phase separation in droplet interface bilayers
af: Danial, J
Udgivet: (2015)

Imaging dynamic pore-formation processes in droplet-interface bilayers
af: Sengel, JT
Udgivet: (2017)

Bilayer formation with fluorinated amphiphiles and applications in membrane protein studies
af: Raychaudhuri, P
Udgivet: (2013)

Mass photometry enables label-free tracking and mass measurement of single proteins on lipid bilayers
af: Foley, E, et al.
Udgivet: (2021)

Nanoscale measurements of the mechanical properties of lipid bilayers
af: Köcher, P
Udgivet: (2014)

Biophysical studies of membrane protein structure and function
af: Dijkman, P, et al.
Udgivet: (2014)

Enhanced stability and fluidity in droplet on hydrogel bilayers for measuring membrane protein diffusion.
af: Thompson, JR, et al.
Udgivet: (2007)

Multiscale simulations and Markov state models of peripheral membrane proteins
af: Amos, SBTA
Udgivet: (2019)

Anomalous diffusion in artificial lipid bilayers
af: Coker, H
Udgivet: (2018)

Confinement and mobility in artificial lipid bilayer interfaces
af: Ramakrishna, VK
Udgivet: (2019)

Biophysical and magnetic resonance studies of membrane proteins
af: Orwick, M, et al.
Udgivet: (2011)

z-STED Imaging and Spectroscopy to Investigate Nanoscale Membrane Structure and Dynamics: supporting dataset
af: Barbotin, A, et al.
Udgivet: (2020)

Single-cell imaging assay for rapid antimicrobial resistance testing
af: Kyropoulos, J
Udgivet: (2021)

DIB‐BOT: An Open‐Source Hardware Approach for Plate‐Integrated Droplet Interface Bilayer Deposition
af: Alexander F Mason, et al.
Udgivet: (2024-12-01)

Mass Photometry of Membrane Proteins
af: Olerinyova, A, et al.
Udgivet: (2020)

Label-free tracking and mass measurement of single protein complexes on lipid bilayers
af: Foley, EDB
Udgivet: (2022)

Plasma membrane dynamics in immune signalling
af: Schneider, F
Udgivet: (2019)

Lipid dynamics in model lipid bilayers analysed by tirf-based single molecule tracking
af: Matysik, Artur
Udgivet: (2015)

Validating an artificial organelle: Studies of lipid droplet-specific proteins on adiposome platform
af: Xuejing Ma, et al.
Udgivet: (2021-08-01)

Controlling Bilayer Curvature and Membrane Protein Density using Droplet Interface Bilayers
af: Castell, O, et al.
Udgivet: (2013)

An investigation of the penetration of lipids in the bilayer of stratum corneum
af: Suhaimi , Hamdan, et al.
Udgivet: (1989)

Stacks of monogalactolipid bilayers can transform into a lattice of water channels
af: Jakub Hryc, et al.
Udgivet: (2023-10-01)

Measuring microtubule binding kinetics of membrane-bound kinesin motors using supported lipid bilayers
af: Rui Jiang, et al.
Udgivet: (2021-09-01)

Biophysical analysis of membrane proteins : investigating structure and function /
af: Pebay-Peyroula, Eva
Udgivet: (2008)

Producing medically important human membrane proteins for structural studies.
af: How, Jonathan Guan Zhong.
Udgivet: (2010)

Studying protein-DNA interactions in vitro and in vivo using single-molecule photoswitching
af: Uphoff, S
Udgivet: (2013)

RNA chain length and stoichiometry govern surface tension and stability of protein-RNA condensates
af: Rabia Laghmach, et al.
Udgivet: (2022-04-01)

Engineered α-hemolysin pores with chemically and genetically-fused functional proteins
af: Mantri, S
Udgivet: (2013)

Engineered α-hemolysin pores with chemically and genetically-fused functional proteins
af: Mantri, S
Udgivet: (2013)

Protocol for analyzing DNA dynamics in the presence of crowders and confined within cell-sized droplets
af: Mehdi Shafiei Aporvari, et al.
Udgivet: (2024-09-01)

Protocol for photo-controlling the assembly of cyclic peptide nanotubes in solution and inside microfluidic droplets
af: Marcos Vilela-Picos, et al.
Udgivet: (2024-06-01)

Pushing the boundaries: molecular dynamics simulations of complex biological membranes
af: Parton, D
Udgivet: (2011)

DNA motor-protein hybrids for molecular transport and self-organisation
af: Wollman, AJM
Udgivet: (2013)

A new detergent-free approach to solubilize membrane proteins to maintain their native environment
af: Bada-Juarez, J
Udgivet: (2018)

phoP maintains the environmental persistence and virulence of pathogenic bacteria in mechanically stressed desiccated droplets
af: Vishnu Hariharan, et al.
Udgivet: (2023-05-01)

PDMS hydrogel-coated tissue culture plates for studying the impact of substrate stiffness on dendritic cell function
af: Megan Lee, et al.
Udgivet: (2022-06-01)

Structural analysis of silk proteins using x–ray and neutron scattering
af: Greving, I
Udgivet: (2012)