Immunofluorescence microscopy-based detection of ssDNA foci by BrdU in mammalian cells
DNA end resection converts broken ends of double-stranded DNA (dsDNA) to 3′-single-stranded DNA (3′-ssDNA). The extent of resection regulates DNA double-strand break (DSB) repair pathway choice and thereby genomic stability. Here, we characterize an optimized immunofluorescence (IF) microscopy-based...
Main Authors: | Kilgas, S, Kiltie, AE, Ramadan, K |
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Format: | Journal article |
Language: | English |
Published: |
Cell Press
2021
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