Immunofluorescence microscopy-based detection of ssDNA foci by BrdU in mammalian cells
DNA end resection converts broken ends of double-stranded DNA (dsDNA) to 3′-single-stranded DNA (3′-ssDNA). The extent of resection regulates DNA double-strand break (DSB) repair pathway choice and thereby genomic stability. Here, we characterize an optimized immunofluorescence (IF) microscopy-based...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
Cell Press
2021
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