Ligation of double-stranded and single-stranded [Oligo(dT)] DNA by vaccinia virus DNA ligase
Vaccinia virus DNA ligase has been expressed in <em>Escherichia coli</em>, purified, and biochemically characterized. The enzyme ligates double-stranded (ds) DNA substrates with either cohesive or blunt-end termini and the latter reaction is stimulated by PEG. Vaccinia virus DNA ligase c...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
Elsevier
1996
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| Subjects: |