Ligation of double-stranded and single-stranded [Oligo(dT)] DNA by vaccinia virus DNA ligase

Ligation of double-stranded and single-stranded [Oligo(dT)] DNA by vaccinia virus DNA ligase

Vaccinia virus DNA ligase has been expressed in <em>Escherichia coli</em>, purified, and biochemically characterized. The enzyme ligates double-stranded (ds) DNA substrates with either cohesive or blunt-end termini and the latter reaction is stimulated by PEG. Vaccinia virus DNA ligase c...

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Bibliographic Details
Main Authors:	Odell, M, Kerr, S, Smith, G
Format:	Journal article
Language:	English
Published:	Elsevier 1996
Subjects:	Viruses Genetics (medical sciences)

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