Optimised oligonucleotide substrates to assay XPF-ERCC1 nuclease activity for the discovery of DNA repair inhibitors
We report the design and optimisation of novel oligonucleotide substrates for a sensitive fluorescence assay for high-throughput screening and functional studies of the DNA repair enzyme, XPF-ERCC1, with a view to accelerating inhibitor and drug discovery.
Bibliografske podrobnosti
| Main Authors: |
Thomas, A,
Brolih, S,
McGouran, J,
El-Sagheer, A,
Ptchelkine, D,
Jones, M,
McDonald, N,
McHugh, P,
Brown, T |
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| Format: | Journal article
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| Izdano: |
Royal Society of Chemistry
2019
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