Optimised oligonucleotide substrates to assay XPF-ERCC1 nuclease activity for the discovery of DNA repair inhibitors

Optimised oligonucleotide substrates to assay XPF-ERCC1 nuclease activity for the discovery of DNA repair inhibitors

We report the design and optimisation of novel oligonucleotide substrates for a sensitive fluorescence assay for high-throughput screening and functional studies of the DNA repair enzyme, XPF-ERCC1, with a view to accelerating inhibitor and drug discovery.

Detalles Bibliográficos
Main Authors: Thomas, A, Brolih, S, McGouran, J, El-Sagheer, A, Ptchelkine, D, Jones, M, McDonald, N, McHugh, P, Brown, T
Formato: Journal article
Publicado: Royal Society of Chemistry 2019

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